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Literature summary for 1.2.1.16 extracted from
- Mutations affecting the enzymes involved in the utilization of 4-aminobutyric acid as nitrogen source by the yeast Saccharomyces cerevisiae (1985), Eur. J. Biochem., 149, 401-404.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | reactivation of purified enzyme preparation | Saccharomyces cerevisiae |  |
| dithiothreitol | reactivation of purified enzyme preparation | Saccharomyces cerevisiae | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| succinate semialdehyde | concentration above 0.1 mM | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.017 | - |
succinate semialdehyde | - |
Saccharomyces cerevisiae | |
| 0.45 | - |
NAD+ | - |
Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| succinate semialdehyde + NADP+ + H2O | Saccharomyces cerevisiae | pathway for utilization of 4-aminobutyric acid as nitrogen source for Saccharomyces cerevisiae | succinate + NADPH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| polyanionic resin Polyanion S1-HR5/5 | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0017 | - |
wild type, value below, NH4+ used as N-source or uga2 mutant unable to grow on 4-aminobutyric acid, glutamate and 4-aminobutyric acid used as N-source | Saccharomyces cerevisiae |
| 0.0025 | - |
wild type, glutamate used as N-source | Saccharomyces cerevisiae |
| 0.0033 | - |
uga3 mutant unable to grow on 4-aminobutyric acid, glutamate and 4-aminobutyric acid used as N-source | Saccharomyces cerevisiae |
| 0.075 | - |
wild type, NH4+ and 4-aminobutyric acid used as N-source | Saccharomyces cerevisiae |
| 0.125 | - |
uga1 mutant unable to grow on 4-aminobutyric acid, glutamate and 4-aminobutyric acid used as N-source | Saccharomyces cerevisiae |
| 0.38 | - |
wild type, glutamate and 4-aminobutyric acid used as N-source | Saccharomyces cerevisiae |
| 0.5 | 0.66 | wild type, 4-aminobutyric acid used as N-source | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| succinate semialdehyde + NAD+ + H2O | - |
Saccharomyces cerevisiae | succinate + NADH + H+ | - |
r | |
| succinate semialdehyde + NADP+ + H2O | - |
Saccharomyces cerevisiae | succinate + NADPH + H+ | - |
? | |
| succinate semialdehyde + NADP+ + H2O | pathway for utilization of 4-aminobutyric acid as nitrogen source for Saccharomyces cerevisiae | Saccharomyces cerevisiae | succinate + NADPH + H+ | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.4 | - |
- |
Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | 2.5fold better activity than with NADP+ at 0.2 mM concentration | Saccharomyces cerevisiae | |
| NADP+ | - |
Saccharomyces cerevisiae | |
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