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Literature summary for 1.2.1.12 extracted from

  • Zhang, C.; Zhuge, B.; Zhan, X.; Fang, H.; Zong, H.; Zhuge, J.
    Cloning and characterization of a novel NAD+-dependent glyceraldehyde-3-phosphate dehydrogenase gene from Candida glycerinogenes and use of its promoter (2013), Yeast, 30, 157-163 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene CgGAP, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain DH5alpha, six stress-response elements are found in the upstream region of the CgGAP gene, phylogenetic analysis, funtional complementation of Saccharomyces cerevisiae transformed with CgGAP and restoration to the wild-type when cultured in high-osmolarity medium. Promoter studies in Saccharomyces cerevisiae using the GFP gene as a reporter show that the GAP promoter (PCgGAP) is constitutively expressed in Saccharomyces cerevisiae cells grown on D-glucose Pichia kudriavzevii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+ Pichia kudriavzevii
-
3-phospho-D-glyceroyl phosphate + NADH + H+
-
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+ Pichia kudriavzevii WL2002-5
-
3-phospho-D-glyceroyl phosphate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Pichia kudriavzevii
-
-
-
Pichia kudriavzevii WL2002-5
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
Pichia kudriavzevii 3-phospho-D-glyceroyl phosphate + NADH + H+
-
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
Pichia kudriavzevii WL2002-5 3-phospho-D-glyceroyl phosphate + NADH + H+
-
?

Subunits

Subunits Comment Organism
More the CgGAP protein consists of an N-terminal NAD+-binding domain and a central catalytic domain Pichia kudriavzevii

Synonyms

Synonyms Comment Organism
CgGAP
-
Pichia kudriavzevii
NAD+-dependent glyceraldehyde-3-phosphate dehydrogenase
-
Pichia kudriavzevii

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pichia kudriavzevii

General Information

General Information Comment Organism
metabolism glyceraldehyde-3-phosphate dehydrogenase, GAPDH, is a key enzyme of glycolysis Pichia kudriavzevii