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Literature summary for 1.2.1.12 extracted from

  • Ringel, A.E.; Ryznar, R.; Picariello, H.; Huang, K.L.; Lazarus, A.G.; Holmes, S.G.
    Yeast Tdh3 (glyceraldehyde 3-phosphate dehydrogenase) is a Sir2-interacting factor that regulates transcriptional silencing and rDNA recombination (2013), PLoS Genet., 9, e1003871 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.12

Protein Variants

Protein Variants Comment Organism
C150G the Tdh3 mutation eliminates catalytic activity, the mutant is defective in silencing and deficient in NAD+ binding Saccharomyces cerevisiae
additional information nuclear export sequences are fused to the 3' end of gene TDH3 by transforming a DNA fragment with 3' homology to the TDH3 ORF, the nuclear export sequence, and an hphMX4 sequence into the appropriate yeast strain. Strains lacking both TDH3 and specific NAD+ biosynthetic genes are generated by crossing Dtdh3 strain YSH969 with selected strains from the yeast deletion collection. Identification of sporulation haploid strains Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Saccharomyces cerevisiae 5737
-
nucleus
-
 Saccharomyces cerevisiae 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+ Saccharomyces cerevisiae
-
 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NAD+ Saccharomyces cerevisiae YSH503
-
 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae YSH503
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Saccharomyces cerevisiae 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Saccharomyces cerevisiae YSH503 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
glyceraldehyde 3-phosphate dehydrogenase
-
 Saccharomyces cerevisiae
TDH3
-
 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction TDH3 deletion or overexpression does not affect overall cellular NAD+ levels, but affects nuclear NAD+ levels in yeast Saccharomyces cerevisiae
physiological function Tdh3, an NAD+-binding protein, influences nuclear NAD+ levels. Tdh3 links nuclear Sir2 with NAD+ from the cytoplasm Saccharomyces cerevisiae