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Literature summary for 1.2.1.12 extracted from

  • Ferreira, E.; Gimenez, R.; Canas, M.A.; Aguilera, L.; Aguilar, J.; Badia, J.; Baldoma, L.
    Glyceraldehyde-3-phosphate dehydrogenase is required for efficient repair of cytotoxic DNA lesions in Escherichia coli (2015), Int. J. Biochem. Cell Biol., 60, 202-212 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene gapA, recombinant expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Escherichia coli

Protein Variants

Protein Variants Comment Organism
C149A site-directed mutagenesis Escherichia coli
C153A site-directed mutagenesis Escherichia coli
additional information construction of a gapA-deficient strain MC4100 DELTAgapA Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+ Escherichia coli
-
3-phospho-D-glyceroyl phosphate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
Escherichia coli 3-phospho-D-glyceroyl phosphate + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
GapA
-
Escherichia coli
GAPDH
-
Escherichia coli
glyceraldehyde-3-phosphate dehydrogenase
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Escherichia coli

General Information

General Information Comment Organism
malfunction GAPDH-deficient cells are more sensitive to bleomycin or methyl methanesulfonate. In cells challenged with these genotoxic agents, GAPDH deficiency results in reduced cell viability and filamentous growth Escherichia coli
metabolism GAPDH is a component of a multiprotein complex that repairs DNA lesions through the base excision repair pathway Escherichia coli
physiological function GAPDH is required for the efficient repair of DNA lesions in Escherichia coli. Interaction occur between GAPDH and enzymes of the base excision repair pathway, namely the AP-endonuclease Endo IV and uracil DNA glycosylase. GAPDH is a component of a protein complex dedicated to the maintenance of genomic DNA integrity. Interaction of GAPDH with the single-stranded DNA binding protein may recruit GAPDH to the repair sites and implicates GAPDH in DNA repair pathways activated by profuse DNA damage, such as homologous recombination or the SOS response. Escherichia coli