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Literature summary for 1.2.1.12 extracted from

  • Maurer, J.; Bovo, F.; Gomes, E.; Loureiro, H.; Stevan, F.; Zawadzki-Baggio, S.; Nakano, M.
    Kinetic data of D-glyceraldehyde-3-phosphate dehydrogenase from HeLa cells (2015), Curr. Enzyme Inhib., 11, 124-131 .
No PubMed abstract available
Search for more literature for 1.2.1.12

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol highest activation at 1 mM Homo sapiens
dithioerythritol highest activation at 1 mM Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate complete inhibition at 10 mM Homo sapiens
4-hydroxymercuribenzoate complete inhibition at 10 mM Homo sapiens
5'-AMP
-
 Homo sapiens
ADP
-
 Homo sapiens
cAMP about 80% inhibition at 30 mM, competitive versus NAD+ Homo sapiens
Cu2+ complete inhibition at 10 mM Homo sapiens
D-glyceraldehyde 3-phosphate 2-mercaptoethanol protects against the inhibition Homo sapiens
Hg2+ complete inhibition at 10 mM Homo sapiens
NADH 2-mercaptoethanol protects against the inhibition, but the inhibitory effect of NADH is not influenced by heavy metal ions or EDTA Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+ Homo sapiens
-
 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Homo sapiens P04406
-
-

Purification (Commentary)

Purification (Comment) Organism
from HeLa cells Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Homo sapiens 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 r

Synonyms

Synonyms Comment Organism
D-glyceraldehyde-3-phosphate dehydrogenase
-
 Homo sapiens
GAPDH
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Homo sapiens
NADH
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.6
-
 cAMP pH and temperature not specified in the publication Homo sapiens
1
-
 5'-AMP pH and temperature not specified in the publication Homo sapiens
2.1
-
 ADP pH and temperature not specified in the publication Homo sapiens

General Information

General Information Comment Organism
metabolism the enzyme is involved in glycolysis, the glycolytic conversion of glucose to pyruvic acid Homo sapiens
physiological function the enzyme is involved in glycolysis, the pathway plays an important role in tumor cells Homo sapiens