Application | Comment | Organism |
---|---|---|
agriculture | cadmium-induced stress in seedlings roots induces nitric oxide accumulation, cytosolic oxidation, activation of the GAPC1 promoter, GAPC1 protein accumulation in enzymatically inactive form, and strong relocalization of GAPC1 to the nucleus. All the effects are detected in the same zone of the root tip. In vitro, GAPC1 is inactivated by either nitric oxide donors or hydrogen peroxide, but no inhibition is directly provided by cadmium | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
C155S | nuclear relocalization of GAPC1 under cadmium-induced oxidative stress is stimulated, rather than inhibited, by mutation of the catalytic cysteine C155 | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | isoform GAPC1 is irreversibly inhibited in a time- and concentration-dependent manner | Arabidopsis thaliana | |
nitric oxide | isoform GAPC1 is irreversibly inhibited in a time- and concentration-dependent manner | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Arabidopsis thaliana | 5829 | - |
nucleus | relocalization of GAPC1 to the nucleus is induced by calcium stress | Arabidopsis thaliana | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Arabidopsis thaliana | - |
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
ecotype Columbia | - |
Arabidopsis thaliana | P25858 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Arabidopsis thaliana | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GapC1 | - |
Arabidopsis thaliana |
GapC1 | isoform | Arabidopsis thaliana |
GapC2 | isoform | Arabidopsis thaliana |
GAPDH | - |
Arabidopsis thaliana |
NAD-dependent glyceraldehyde-3-phosphate dehydrogenase | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | 72 h cadmium treatment has no stimulating effect on the extractable NADH-specific glyceraldehyde-3-phosphate dehydrogenase activity of whole seedlings | additional information |
Arabidopsis thaliana | cadmium induces isoform GAPC1 accumulation in an inactive form. L-buthionine-sulfoximine induces both isoform GAPC1 promoter activity and protein accumulation in the seedling root tips | up |
General Information | Comment | Organism |
---|---|---|
physiological function | cadmium-induced stress in seedlings roots induces nitric oxide accumulation, cytosolic oxidation, activation of the GAPC1 promoter, GAPC1 protein accumulation in enzymatically inactive form, and strong relocalization of GAPC1 to the nucleus. All the effects are detected in the same zone of the root tip. In vitro, GAPC1 is inactivated by either nitric oxide donors or hydrogen peroxide, but no inhibition is directly provided by cadmium | Arabidopsis thaliana |