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Literature summary for 1.2.1.12 extracted from

  • Mukherjee, S.; Saha, B.; Dutta, D.; Das, A.
    Purification, crystallization and preliminary X-ray analysis of apo glyceraldehyde-3-phosphate dehydrogenase 1 (GAP1) from methicillin-resistant Staphylococcus aureus (MRSA252) (2010), Acta Crystallogr. Sect. F, 66, 506-508.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli M15 cells Staphylococcus aureus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.1 M Tris-HCl pH 8.2, 28% (w/v) PEG 3350, at 25°C Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+ Staphylococcus aureus
-
3-phospho-D-glyceroyl phosphate + NADH
-
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+ Staphylococcus aureus MRSA252
-
3-phospho-D-glyceroyl phosphate + NADH
-
?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus Q6GIL8
-
-
Staphylococcus aureus MRSA252 Q6GIL8
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose column chromatography and Superdex 75 gel filtration Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
Staphylococcus aureus 3-phospho-D-glyceroyl phosphate + NADH
-
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
Staphylococcus aureus MRSA252 3-phospho-D-glyceroyl phosphate + NADH
-
?

Subunits

Subunits Comment Organism
homotetramer x-ray crystallography Staphylococcus aureus

Synonyms

Synonyms Comment Organism
GAP1
-
Staphylococcus aureus
glyceraldehyde-3-phosphate dehydrogenase 1
-
Staphylococcus aureus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Staphylococcus aureus