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Literature summary for 1.2.1.12 extracted from

  • Arutiunova, E.I.; Pleten, A.P.; Nagradova, N.K.; Muronetz, V.I.
    Antibodies to inactive conformations of glyceraldehyde-3-phosphate dehydrogenase inactivate the apo- and holoforms of the enzyme (2006), Biochemistry (Moscow), 71, 685-691.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloned into the pBluescript vector for expression in Escherichia coli GM-109 cells Geobacillus stearothermophilus

Inhibitors

Inhibitors Comment Organism Structure
additional information the produced type I antibody induces a time-dependent decrease in the activity by 80-90% of the active holoenzyme and 25% of the apoenzyme Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+ Geobacillus stearothermophilus activity assay 3-phospho-D-glyceroyl phosphate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using gel filtration on a AcA-34 column, and a Q-Sepharose and phenyl-Sepharose column Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
150
-
purified enzyme Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+ activity assay Geobacillus stearothermophilus 3-phospho-D-glyceroyl phosphate + NADH + H+
-
?

Subunits

Subunits Comment Organism
tetramer
-
Geobacillus stearothermophilus

Synonyms

Synonyms Comment Organism
GAPDH
-
Geobacillus stearothermophilus
glyceraldehyde-3-phosphate dehydrogenase
-
Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
activity assay Geobacillus stearothermophilus

Cofactor

Cofactor Comment Organism Structure
NADH
-
Geobacillus stearothermophilus