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Literature summary for 1.2.1.11 extracted from

  • Evitt, A.S.; Cox, R.J.
    Synthesis and evaluation of conformationally restricted inhibitors of aspartate semialdehyde dehydrogenase (2011), Mol. Biosyst., 7, 1564-1575.
    View publication on PubMed
Search for more literature for 1.2.1.11

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Salmonella enterica

Inhibitors

Inhibitors Comment Organism Structure
cis-5-phosphonic acid pipecolic acid
-
 Salmonella enterica
pipecolic acid-5-(R)-phosphate hydrochloric acid
-
 Salmonella enterica
pipecolic acid-5-(S)-phosphate hydrochloric acid
-
 Salmonella enterica
trans-5-phosphonic acid pipecolic acid
-
 Salmonella enterica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
 phosphate in 0.2 M bicine buffer, pH 8.6, at 25°C Salmonella enterica
0.16
-
 L-4-aspartyl phosphate in 0.2 M bicine buffer, pH 8.6, at 25°C Salmonella enterica
0.17
-
 L-aspartate 4-semialdehyde in 0.2 M bicine buffer, pH 8.6, at 25°C Salmonella enterica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 2 * 39000, SDS-PAGE Salmonella enterica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-4-aspartyl phosphate + NADPH + H+ Salmonella enterica
-
 L-aspartate 4-semialdehyde + phosphate + NADP+
-
 r
L-aspartate 4-semialdehyde + phosphate + NADP+ Salmonella enterica
-
 L-4-aspartyl phosphate + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Salmonella enterica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Salmonella enterica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-4-aspartyl phosphate + NADPH + H+
-
 Salmonella enterica L-aspartate 4-semialdehyde + phosphate + NADP+
-
 r
L-aspartate 4-semialdehyde + phosphate + NADP+
-
 Salmonella enterica L-4-aspartyl phosphate + NADPH + H+
-
 r

Subunits

Subunits Comment Organism
homodimer 2 * 39000, SDS-PAGE Salmonella enterica

Synonyms

Synonyms Comment Organism
ASADH
-
 Salmonella enterica
aspartate semialdehyde dehydrogenase
-
 Salmonella enterica

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Salmonella enterica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
236
-
 L-aspartate 4-semialdehyde in 0.2 M bicine buffer, pH 8.6, at 25°C Salmonella enterica
265
-
 phosphate in 0.2 M bicine buffer, pH 8.6, at 25°C Salmonella enterica