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Literature summary for 1.2.1.11 extracted from

  • Tunca, S.; Yilmaz, E.I.; Piret, J.; Liras, P.; Ozcengiz, G.
    Cloning, characterization and heterologous expression of the aspartokinase and aspartate semialdehyde dehydrogenase genes of cephamycin C-producer Streptomyces clavuligerus (2004), Res. Microbiol., 155, 525-534.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene asd, cloning from genomic DNA library, DNA sequence determination and analysis, expression in enzyme-deficient auxotrophic Escherichia coli strain CGSC5080 Streptomyces clavuligerus

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by pathway endproducts amino acids threonine, methionine, lysine, and isoleucine Streptomyces clavuligerus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-4-aspartyl phosphate + NADPH Streptomyces clavuligerus 2nd step in the biosynthetic aspartate pathway, also required for biosynthesis of the beta-lactam caphamycin, overview L-aspartate-4-semialdehyde + phosphate + NADP+
-
?
L-4-aspartyl phosphate + NADPH Streptomyces clavuligerus NRRL 3585 2nd step in the biosynthetic aspartate pathway, also required for biosynthesis of the beta-lactam caphamycin, overview L-aspartate-4-semialdehyde + phosphate + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces clavuligerus Q8KQ27 gene asd
-
Streptomyces clavuligerus NRRL 3585 Q8KQ27 gene asd
-

Purification (Commentary)

Purification (Comment) Organism
partially, recombinant enzyme from Escherichia coli, 37fold Streptomyces clavuligerus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.373
-
partially purified recombinant enzyme Streptomyces clavuligerus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-4-aspartyl phosphate + NADPH
-
Streptomyces clavuligerus L-aspartate-4-semialdehyde + phosphate + NADP+
-
?
L-4-aspartyl phosphate + NADPH 2nd step in the biosynthetic aspartate pathway, also required for biosynthesis of the beta-lactam caphamycin, overview Streptomyces clavuligerus L-aspartate-4-semialdehyde + phosphate + NADP+
-
?
L-4-aspartyl phosphate + NADPH
-
Streptomyces clavuligerus NRRL 3585 L-aspartate-4-semialdehyde + phosphate + NADP+
-
?
L-4-aspartyl phosphate + NADPH 2nd step in the biosynthetic aspartate pathway, also required for biosynthesis of the beta-lactam caphamycin, overview Streptomyces clavuligerus NRRL 3585 L-aspartate-4-semialdehyde + phosphate + NADP+
-
?

Synonyms

Synonyms Comment Organism
aspartate semialdehyde dehydrogenase
-
Streptomyces clavuligerus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Streptomyces clavuligerus