Cloned (Comment) | Organism |
---|---|
gene asd, cloning from genomic DNA library, DNA sequence determination and analysis, expression in enzyme-deficient auxotrophic Escherichia coli strain CGSC5080 | Streptomyces clavuligerus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by pathway endproducts amino acids threonine, methionine, lysine, and isoleucine | Streptomyces clavuligerus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-4-aspartyl phosphate + NADPH | Streptomyces clavuligerus | 2nd step in the biosynthetic aspartate pathway, also required for biosynthesis of the beta-lactam caphamycin, overview | L-aspartate-4-semialdehyde + phosphate + NADP+ | - |
? | |
L-4-aspartyl phosphate + NADPH | Streptomyces clavuligerus NRRL 3585 | 2nd step in the biosynthetic aspartate pathway, also required for biosynthesis of the beta-lactam caphamycin, overview | L-aspartate-4-semialdehyde + phosphate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces clavuligerus | Q8KQ27 | gene asd | - |
Streptomyces clavuligerus NRRL 3585 | Q8KQ27 | gene asd | - |
Purification (Comment) | Organism |
---|---|
partially, recombinant enzyme from Escherichia coli, 37fold | Streptomyces clavuligerus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.373 | - |
partially purified recombinant enzyme | Streptomyces clavuligerus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-4-aspartyl phosphate + NADPH | - |
Streptomyces clavuligerus | L-aspartate-4-semialdehyde + phosphate + NADP+ | - |
? | |
L-4-aspartyl phosphate + NADPH | 2nd step in the biosynthetic aspartate pathway, also required for biosynthesis of the beta-lactam caphamycin, overview | Streptomyces clavuligerus | L-aspartate-4-semialdehyde + phosphate + NADP+ | - |
? | |
L-4-aspartyl phosphate + NADPH | - |
Streptomyces clavuligerus NRRL 3585 | L-aspartate-4-semialdehyde + phosphate + NADP+ | - |
? | |
L-4-aspartyl phosphate + NADPH | 2nd step in the biosynthetic aspartate pathway, also required for biosynthesis of the beta-lactam caphamycin, overview | Streptomyces clavuligerus NRRL 3585 | L-aspartate-4-semialdehyde + phosphate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aspartate semialdehyde dehydrogenase | - |
Streptomyces clavuligerus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Streptomyces clavuligerus |