Literature summary for 1.2.1.11 extracted from

Nichols, C.E.; Dhaliwal, B.; Lockyer, M.; Hawkins, A.R.; Stammers, D.K.
High-resolution structures reveal details of domain closure and "half-of-sites-reactivity" in Escherichia coli aspartate beta-semialdehyde dehydrogenase (2004), J. Mol. Biol., 341, 797-806.

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Application

Application	Comment	Organism
medicine	enzyme is potential target for antimicrobial drugs	Escherichia coli

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant large scale expression in strain BL21(DE3)	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme in open and closed form, 30 mg/ml purified recombinant enzyme in 10 mM Tris, pH 7.4, 40 mM KCl, with equal volume of reservoir solution, 0.006 ml sitting drops by vapour diffusion utilizing micro-bridges, 20% v/v ethylene glycol, 4°C, X-ray diffraction structure determination and analysis at 1.95 A and 1.6 A resolution, respectively, modeling	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-4-aspartyl phosphate + NADPH	Escherichia coli	part of the biosynthetic aspartate pathway	L-aspartate-4-semialdehyde + phosphate + NADP+	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0A9Q9	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from strain BL21(DE3) in several steps	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-4-aspartyl phosphate + NADPH	part of the biosynthetic aspartate pathway	Escherichia coli	L-aspartate-4-semialdehyde + phosphate + NADP+	-	?
L-4-aspartyl phosphate + NADPH	reductive dephosphorylation	Escherichia coli	L-aspartate-4-semialdehyde + phosphate + NADP+	-	?

Subunits

Subunits	Comment	Organism
dimer	crystal structure, subdomain structure of subunits, open and closed enzyme forms	Escherichia coli

Synonyms

Synonyms	Comment	Organism
aspartate beta-semialdehyde dehydrogenase	-	Escherichia coli
ecASADH	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Escherichia coli

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Release 2023.1 (January 2023)