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Literature summary for 1.2.1.11 extracted from

  • Preiss, J.; Mazelis, M.; Greenberg, E.
    Cloning of the aspartate-beta-semialdehyde dehydrogenase structural gene from Escherichia coli K12 (1982), Curr. Microbiol., 7, 263-268.
No PubMed abstract available
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Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 L-aspartate 4-semialdehyde
-
 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 2 * 40000 Escherichia coli
77000
-
 sucrose density gradient centrifugation Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 K-12
-

Purification (Commentary)

Purification (Comment) Organism
using heat treatment, column chromatography on DEAE-cellulose, ammonium sulfate precipitation and column chromatography on Sephadex G-200 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.002
-
 K12 6201 strain, enriched media plus diaminopimelic acid Escherichia coli
0.12
-
 B AC70R1 strain, enriched media Escherichia coli
0.4
-
 K12 23631 strain, minimal media plus N-formyllysine Escherichia coli
0.94
-
 B AC70R1 strain, minimal media Escherichia coli
1.8
-
 K12 6201/PoP126 strain, enriched media plus ampicillin Escherichia coli
2.7
-
 B AC70R1/PoP12 strain, enriched media plus tetracycline Escherichia coli
5.3
-
 B AC70R1/PoP12 strain, minimal media plus tetracycline Escherichia coli
26
-
 K12 23631/pOP12 strain, minimal media plus N-formyllysine and tetracycline Escherichia coli
145
-
 K12 23631/pOP12, after three times purification of the enzyme Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate 4-semialdehyde + phosphate + NADP+
-
 Escherichia coli L-4-aspartyl phosphate + NADPH
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 40000 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Escherichia coli