Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.1.11 extracted from

  • Holland, M.J.; Westhead, E.W.
    Purification and characterization of aspartic -semialdehyde dehydrogenase from yeast and purification of an isozyme of glyceraldehyde-3-phosphate dehydrogenase (1973), Biochemistry, 12, 2264-2270.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.036
-
NADP+
-
Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
4 * 41000, SDS-PAGE Saccharomyces cerevisiae
156000
-
sedimentation equilibrium, meniscus depletion technique Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-4-aspartyl phosphate + NADPH Saccharomyces cerevisiae synthesis of threonine L-aspartate 4-semialdehyde + phosphate + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using protamine sulfate treatment, ammonium sulfate precipitation, chromatography on DEAE-Sephadex A-50 column, Bio-Gel hydroxylapatite phosphate column, Bio-Gel hydroxylapatite sulfate column, acid treatment and column chromatography on Sephadex G-200 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
73
-
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-4-aspartyl phosphate + NADPH synthesis of threonine Saccharomyces cerevisiae L-aspartate 4-semialdehyde + phosphate + NADP+
-
?
L-aspartate 4-semialdehyde + phosphate + NADP+
-
Saccharomyces cerevisiae L-4-aspartyl phosphate + NADPH
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 41000, SDS-PAGE Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Saccharomyces cerevisiae