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Literature summary for 1.2.1.10 extracted from

  • Inokuma, K.; Nakashimada, Y.; Akahoshi, T.; Nishio, N.
    Characterization of enzymes involved in the ethanol production of Moorella sp. HUC22-1 (2007), Arch. Microbiol., 188, 37-45.
    View publication on PubMed
Search for more literature for 1.2.1.10

Application

Application Comment Organism
synthesis 50 microg of alcohol dehydrogenase AdhA, EC 1.1.1.2, and 50 microg actaldehyde dehydrogenase AldH, EC 1.2.1.10, in buffer solution (pH 8.0) containing NADPH, NADH and acetyl-CoA at 60°C, produce 1.6 mM ethanol from 3 mM acetyl-CoA after 90 min Moorella sp. HUC22-1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Moorella sp. HUC22-1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6
-
 acetyl-CoA pH 8.0, 60°C Moorella sp. HUC22-1
4.3
-
 acetaldehyde pH 8.0, 60°C Moorella sp. HUC22-1

Organism

Organism UniProt Comment Textmining
Moorella sp. HUC22-1
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8
-
 cleavage of thioester, cofactor NADPH, pH 8.0, 60°C Moorella sp. HUC22-1
3.7
-
 condensation of thioester, cofactor NADP+, pH 10.0, 60°C Moorella sp. HUC22-1
18.8
-
 cleavage of thioester, cofactor NADH, pH 8.0, 60°C Moorella sp. HUC22-1
29.9
-
 condensation of thioester, cofactor NAD+, pH 10.0, 60°C Moorella sp. HUC22-1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NAD+ + CoA
-
 Moorella sp. HUC22-1 acetyl-CoA + NADH + H+
-
 r
acetaldehyde + NADP+ + CoA
-
 Moorella sp. HUC22-1 acetyl-CoA + NADPH + H+
-
 r
acetyl-CoA + NADH + H+
-
 Moorella sp. HUC22-1 acetaldehyde + NAD+ + CoA
-
 r
acetyl-CoA + NADPH + H+
-
 Moorella sp. HUC22-1 acetaldehyde + NADP+ + CoA
-
 r

Synonyms

Synonyms Comment Organism
ALDH
-
 Moorella sp. HUC22-1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 at pH 8.0 Moorella sp. HUC22-1

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50
-
 71% of maximum activity Moorella sp. HUC22-1
70
-
 52% of maximum activity Moorella sp. HUC22-1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 cleavage of thioester Moorella sp. HUC22-1
10
-
 condensation of thiopester Moorella sp. HUC22-1

Cofactor

Cofactor Comment Organism Structure
NADH NADH is preferred 7-8fold over NADPH Moorella sp. HUC22-1
NADPH NADH is preferred 7-8fold over NADPH Moorella sp. HUC22-1