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Literature summary for 1.2.1.10 extracted from

  • Yan, R.T.; Chen, J.S.
    Coenzyme A-acylating aldehyde dehydrogenase from Clostridium beijerinckii NRRL B592 (1990), Appl. Environ. Microbiol., 56, 2591-2599.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.10

General Stability

General Stability Organism
sensitive to O2, can be protected against O2 inactivation by dithiothreitol Clostridium beijerinckii
with Tris acetate buffer at pH 7, less stable at higher buffer concentration between 10 and 150 mM Clostridium beijerinckii

Inhibitors

Inhibitors Comment Organism Structure
NAD+ double competitive, NAD+/CoA ratio is kept at 1:0.24 Clostridium beijerinckii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0076
-
 NADH forward reaction, with butyryl-CoA Clostridium beijerinckii
0.0082
-
 NADH forward reaction, with acetyl-CoA Clostridium beijerinckii
0.0196
-
 acetyl-CoA forward reaction, with NADPH Clostridium beijerinckii
0.064
-
 CoA reverse reaction, with 0.32 mM NAD+ and 11 mM butyraldehyde Clostridium beijerinckii
0.0673
-
 NADPH forward reaction, with butyryl-CoA Clostridium beijerinckii
0.0715
-
 n-butyryl-CoA forward reaction, with NADPH Clostridium beijerinckii
0.154
-
 acetyl-CoA forward reaction, with NADH Clostridium beijerinckii
0.166
-
 n-butyryl-CoA forward reaction, with NADH Clostridium beijerinckii
0.206
-
 NADPH forward reaction, with acetyl-CoA Clostridium beijerinckii
0.25
-
 NAD+ reverse reaction, with 0.12 mM CoA and 11 mM butyraldehyde Clostridium beijerinckii
3.7
-
 Butyraldehyde reverse reaction, with 0.32 mM NAD+ and 0.12 mM CoA Clostridium beijerinckii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 2 * 55000, SDS-PAGE Clostridium beijerinckii
100000
-
 gel filtration under anaerobic conditions Clostridium beijerinckii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetaldehyde + CoA + NAD+ Clostridium beijerinckii
-
 acetyl-CoA + NADH
-
 r
acetaldehyde + CoA + NAD+ Clostridium beijerinckii NRRL B592
-
 acetyl-CoA + NADH
-
 r

Organism

Organism UniProt Comment Textmining
Clostridium beijerinckii
-
-
-
Clostridium beijerinckii NRRL B592
-
-
-

Purification (Commentary)

Purification (Comment) Organism
under anaerobic conditions Clostridium beijerinckii

Reaction

Reaction Comment Organism Reaction ID
acetaldehyde + CoA + NAD+ = acetyl-CoA + NADH + H+ bi-uni-uni-uni-ping-pong mechanism Clostridium beijerinckii
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2
-
-
 Clostridium beijerinckii
4.88
-
 undialyzed, anaerobically purified Clostridium beijerinckii
9.62
-
 dialyzed, incubated with dithiothreitol and CoA Clostridium beijerinckii

Storage Stability

Storage Stability Organism
5 mM dithiothreitol protects enzyme in crude extracts from O2-inactivation for at least 2 hours Clostridium beijerinckii
addition of 140 mM KCl to 10 mM Tris acetate stabilizes as no activity is lost after 3 days Clostridium beijerinckii
CoA and dithiothreitol restore a higher activity than one of these compounds alone Clostridium beijerinckii
glycerol at 20%, vol/vol, stabilizes in 50 mM Tris acetate buffer at pH 7, as no activity is lost after 3 days at 4°C under argon Clostridium beijerinckii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + CoA + NAD+
-
 Clostridium beijerinckii acetyl-CoA + NADH
-
 r
acetaldehyde + CoA + NAD+
-
 Clostridium beijerinckii NRRL B592 acetyl-CoA + NADH
-
 r
acetyl-CoA + NADPH + H+
-
 Clostridium beijerinckii acetaldehyde + CoA + NADP+
-
 r
acetyl-CoA + NADPH + H+
-
 Clostridium beijerinckii NRRL B592 acetaldehyde + CoA + NADP+
-
 r
butyryl-CoA + NADPH + H+
-
 Clostridium beijerinckii butyraldehyde + CoA + NADP+
-
 r
butyryl-CoA + NADPH + H+
-
 Clostridium beijerinckii NRRL B592 butyraldehyde + CoA + NADP+
-
 r
n-butyraldehyde + CoA + NAD+
-
 Clostridium beijerinckii n-butyryl-CoA + NADH
-
 r
n-butyraldehyde + CoA + NAD+
-
 Clostridium beijerinckii NRRL B592 n-butyryl-CoA + NADH
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 55000, SDS-PAGE Clostridium beijerinckii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Clostridium beijerinckii

pH Range

pH Minimum pH Maximum Comment Organism
6 7 NAD+ reduction Clostridium beijerinckii
6.5 9.5 activity of NADH oxidation increases between 6.5-9.5 Clostridium beijerinckii

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
 more stable at pH 7 than at pH 6, 8 or 9 in either Tris acetate or potassium phosphate test buffer, at the same pH more stable in Tris acetate test buffer Clostridium beijerinckii

Cofactor

Cofactor Comment Organism Structure
CoA
-
 Clostridium beijerinckii
NAD+
-
 Clostridium beijerinckii
NADH shows also activity with NADPH, NADH is more effective than NADPH Clostridium beijerinckii