KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
n-butyryl-CoA | - |
Thermoanaerobacter ethanolicus | |
0.037 | - |
acetyl-CoA | - |
Thermoanaerobacter ethanolicus | |
0.063 | - |
NADH | - |
Thermoanaerobacter ethanolicus | |
0.16 | - |
CoA | - |
Thermoanaerobacter ethanolicus | |
0.44 | - |
NAD+ | - |
Thermoanaerobacter ethanolicus | |
1.3 | - |
acetaldehyde | - |
Thermoanaerobacter ethanolicus | |
2.3 | - |
Butanal | - |
Thermoanaerobacter ethanolicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
4 * 100000, SDS-PAGE | Thermoanaerobacter ethanolicus |
360000 | - |
gel filtration | Thermoanaerobacter ethanolicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + CoA + NAD+ | Thermoanaerobacter ethanolicus | - |
acetyl-CoA + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoanaerobacter ethanolicus | - |
- |
- |
Thermoanaerobacter ethanolicus 39E | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermoanaerobacter ethanolicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16 | - |
- |
Thermoanaerobacter ethanolicus |
Storage Stability | Organism |
---|---|
4°C, anaerobically | Thermoanaerobacter ethanolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + CoA + NAD+ | - |
Thermoanaerobacter ethanolicus | acetyl-CoA + NADH | - |
r | |
n-butyraldehyde + CoA + NAD+ | - |
Thermoanaerobacter ethanolicus | n-butyryl-CoA + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 100000, SDS-PAGE | Thermoanaerobacter ethanolicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
thioesterase activity | Thermoanaerobacter ethanolicus |
8.5 | - |
NAD+ reduction | Thermoanaerobacter ethanolicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
CoA | - |
Thermoanaerobacter ethanolicus | |
NAD+ | NADH-dependent | Thermoanaerobacter ethanolicus | |
NADH | - |
Thermoanaerobacter ethanolicus |