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Literature summary for 1.2.1.10 extracted from

  • Burdette, D.; Zeikus, J.G.
    Purification of acetaldehyde dehydrogenase and alcohol dehydrogenases from Thermoanaerobacter ethanolicus 39E and characterization of the secondary-alcohol dehydrogenase (2 degrees Adh) as a bifunctional alcohol dehydrogenase-acetyl-CoA reductive thioesterase (1994), Biochem. J., 302, 163-170.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
n-butyryl-CoA
-
Thermoanaerobacter ethanolicus
0.037
-
acetyl-CoA
-
Thermoanaerobacter ethanolicus
0.063
-
NADH
-
Thermoanaerobacter ethanolicus
0.16
-
CoA
-
Thermoanaerobacter ethanolicus
0.44
-
NAD+
-
Thermoanaerobacter ethanolicus
1.3
-
acetaldehyde
-
Thermoanaerobacter ethanolicus
2.3
-
Butanal
-
Thermoanaerobacter ethanolicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
4 * 100000, SDS-PAGE Thermoanaerobacter ethanolicus
360000
-
gel filtration Thermoanaerobacter ethanolicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetaldehyde + CoA + NAD+ Thermoanaerobacter ethanolicus
-
acetyl-CoA + NADH
-
r

Organism

Organism UniProt Comment Textmining
Thermoanaerobacter ethanolicus
-
-
-
Thermoanaerobacter ethanolicus 39E
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermoanaerobacter ethanolicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16
-
-
Thermoanaerobacter ethanolicus

Storage Stability

Storage Stability Organism
4°C, anaerobically Thermoanaerobacter ethanolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + CoA + NAD+
-
Thermoanaerobacter ethanolicus acetyl-CoA + NADH
-
r
n-butyraldehyde + CoA + NAD+
-
Thermoanaerobacter ethanolicus n-butyryl-CoA + NADH
-
r

Subunits

Subunits Comment Organism
tetramer 4 * 100000, SDS-PAGE Thermoanaerobacter ethanolicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
thioesterase activity Thermoanaerobacter ethanolicus
8.5
-
NAD+ reduction Thermoanaerobacter ethanolicus

Cofactor

Cofactor Comment Organism Structure
CoA
-
Thermoanaerobacter ethanolicus
NAD+ NADH-dependent Thermoanaerobacter ethanolicus
NADH
-
Thermoanaerobacter ethanolicus