Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | several organic acids highly stimulate the enzyme activity of anaerobically isolated bacteroids, only malate has little effect | Bradyrhizobium japonicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced ferredoxin + H+ + N2 + ATP + H2O | Bradyrhizobium japonicum | reduction of atmospheric dinitrogen to ammonium | oxidized ferredoxin + H2 + NH3 + ADP + phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bradyrhizobium japonicum | - |
strains 110 or 2134, anaerobically and aerobically isolated bacteroid from nodules of soybean | - |
Oxidation Stability | Organism |
---|---|
enzyme is extremely oxygen-labile | Bradyrhizobium japonicum |
Renatured (Comment) | Organism |
---|---|
recovery of enzyme activity from aerobically isolated soybean nodules by incubation at low O2 pressure for about 1 h, recovery of activity can be inhibited by chloramphenicol and puromycin, and by spectinomycin only in strain 2134, not in strain 110, cycloheximide, actinomycin D, nalidixic acid, and rifampicin have no effect on enzyme activity recovery in strain 110, and cycloheximide, actinomycin D, and streptomycin have no effect on enzyme activity recovery in strain 2134 | Bradyrhizobium japonicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylene + ? | - |
Bradyrhizobium japonicum | ethylene + ? | - |
? | |
reduced ferredoxin + H+ + N2 + ATP + H2O | - |
Bradyrhizobium japonicum | oxidized ferredoxin + H2 + NH3 + ADP + phosphate | - |
ir | |
reduced ferredoxin + H+ + N2 + ATP + H2O | reduction of atmospheric dinitrogen to ammonium | Bradyrhizobium japonicum | oxidized ferredoxin + H2 + NH3 + ADP + phosphate | - |
ir |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Bradyrhizobium japonicum | |
Ferredoxin | - |
Bradyrhizobium japonicum |