Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ required for MgATP complex | Clostridium pasteurianum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27500 | - |
2 * 27500, SDS-PAGE | Clostridium pasteurianum |
27500 | - |
component I Fe protein 2 * 27500 + component II MoFe protein 1 * 60000 + 1 * 51000, SDS-PAGE | Clostridium pasteurianum |
51000 | - |
component I Fe protein 2 * 27500 + component II MoFe protein 1 * 60000 + 1 * 51000, SDS-PAGE | Clostridium pasteurianum |
56000 | - |
Fe protein, gel filtration | Clostridium pasteurianum |
60000 | - |
component I Fe protein 2 * 27500 + component II MoFe protein 1 * 60000 + 1 * 51000, SDS-PAGE | Clostridium pasteurianum |
210000 | - |
MoFe protein, gel filtration | Clostridium pasteurianum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | Clostridium pasteurianum | - |
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium pasteurianum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
all components | Clostridium pasteurianum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein | Clostridium pasteurianum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | - |
Clostridium pasteurianum | 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 27500, SDS-PAGE | Clostridium pasteurianum |
tetramer | component I Fe protein 2 * 27500 + component II MoFe protein 1 * 60000 + 1 * 51000, SDS-PAGE | Clostridium pasteurianum |