Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | the protein contains 2 g atoms each of iron | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
13600 | - |
x * 13600, SDS-PAGE | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | Q52126 | - |
- |
Pseudomonas putida NCIB 9816 | Q52126 | - |
- |
Purification (Comment) | Organism |
---|---|
Blue Sepharose column chromatography, DEAE-cellulose column chromatography, and Sephadex G-75 gel filtration | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.52 | - |
purified enzyme, in the absence of FAD and with NADPH as cosubstrate, pH and temperature not specified in the publication | Pseudomonas putida |
13.2 | - |
purified enzyme, in the absence of FAD and with NADH as cosubstrate, pH and temperature not specified in the publication | Pseudomonas putida |
36.7 | - |
purified enzyme, in the presence of FAD and NADPH, pH and temperature not specified in the publication | Pseudomonas putida |
71 | - |
purified enzyme, in the presence of FAD and NADH, pH and temperature not specified in the publication | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricytochrome c + NADH | the enzyme has about 2fold higher activity with NADH compared to NADPH | Pseudomonas putida | 2 ferrocytochrome c + NAD+ + H+ | - |
? | |
2 ferricytochrome c + NADH | the enzyme has about 2fold higher activity with NADH compared to NADPH | Pseudomonas putida NCIB 9816 | 2 ferrocytochrome c + NAD+ + H+ | - |
? | |
oxidized cytochrome c + NADPH | - |
Pseudomonas putida | reduced cytochrome c + NADP+ + H+ | - |
? | |
oxidized cytochrome c + NADPH | - |
Pseudomonas putida NCIB 9816 | reduced cytochrome c + NADP+ + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 13600, SDS-PAGE | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
NADH-ferredoxinNAP reductase | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | preferred flavin cofactor | Pseudomonas putida | |
NADH | NADH is preferred over NADPH | Pseudomonas putida | |
NADPH | - |
Pseudomonas putida |