Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Bos taurus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
x * 51000, tightly bound enzyme form, SDS-PAGE | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
the tightly bound enzyme form is purified by sodium cholate solubilization, ammonium sulfate precipitation, DEAE-Sepharose column chromatography, 2,5-ADP-Sepharose column chromatography, and DEAE-Sepharose column chromatography. The loosely bound enzyme form is purified by ammonium sulfate precipitation, DEAE-cellulose column chromatography, 2,5-ADP-Sepharose column chromatography, and Sephadex G-25 gel filtration | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal cortex | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.03 | - |
tightly bound enzyme form from homogenate, at pH 7.4 and 25°C | Bos taurus |
22 | - |
tightly bound enzyme form after 733fold purification, at pH 7.4 and 25°C | Bos taurus |
Storage Stability | Organism |
---|---|
-80°C, 50 mm potassium phosphate buffer (pH 7.4) in the dark, at least 3 months, no loss of activity | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxidized adrenodoxin + NADPH + H+ | tightly and loosely bound enzyme forms show similar activities | Bos taurus | reduced adrenodoxin + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 51000, tightly bound enzyme form, SDS-PAGE | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
adrenodoxin reductase | - |
Bos taurus |
NADPH-adrenodoxin reductase | - |
Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Bos taurus |