Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | sharp decrease in activity in presence of up to 50 mM KCl, smaller increase in inhibition above | Mycobacterium sp. | |
NaCl | sharp decrease in activity in presence of up to 50 mM KCl, smaller increase in inhibition above | Mycobacterium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000037 | - |
oxidized ferredoxin | recombinant ferredoxin with C-terminal His-tag, pH 8.6 | Mycobacterium sp. | |
0.0000053 | - |
oxidized ferredoxin | wild-type ferredoxin, pH 8.6 | Mycobacterium sp. | |
0.0000105 | - |
oxidized ferredoxin | recombinant ferredoxin with N-terminal His-tag, pH 8.6 | Mycobacterium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium sp. | - |
- |
- |
Renatured (Comment) | Organism |
---|---|
reconstitution of P450-containing mono-oxygenase using purified homologous enzyme, ferrdoxin and P450 | Mycobacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricytochrome c + NADH | - |
Mycobacterium sp. | 2 ferrocytochrome c + NAD+ + H+ | - |
? | |
additional information | no substrates of enzyme: morpholine, piperidine, pyrrolidine. Morpholine is a substrate of the P450mor system, consisting of enzyme, Fe3S4 ferredoxin and cytochrome P450 | Mycobacterium sp. | ? | - |
? | |
NADH + nitroblue tetrazolium | - |
Mycobacterium sp. | NAD+ + reduced nitroblue tetrazolium | - |
? | |
oxidized ferredoxin + NADH + H+ | - |
Mycobacterium sp. | reduced ferredoxin + NAD+ | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
- |
Mycobacterium sp. |