Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | Fprs are induced by Fe2+ depletion but not by Fe2+ excess | Pseudomonas putida | |
Fe3+ | 0.5 microM Fe(III)-citrate and 0.5 microM Fe(III)-EDTA induces Fprs | Pseudomonas putida | |
H2O2 | 1.0 mM, oxidative stress induces Pseudomonas putida FprA but not FprB | Pseudomonas putida | |
menadione | 1.0 mM, oxidative stress induces Pseudomonas putida FprA but not FprB | Pseudomonas putida | |
paraquat | 0.5 mM, oxidative stress induces Pseudomonas putida FprA but not FprB | Pseudomonas putida | |
tert-butyl hydroperoxide | 0.5 mM, oxidative stress induces Pseudomonas putida FprA but not FprB | Pseudomonas putida |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00235 | - |
NADH | for FprB with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
0.00273 | - |
NADH | for FprA with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
0.00338 | - |
NADH | for FprB in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
0.00457 | - |
NADPH | for FprA in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
0.00459 | - |
NADPH | for FprA in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
0.00587 | - |
NADPH | for FprB in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
0.00616 | - |
NADH | for FprB with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
0.00625 | - |
NADPH | for FprA with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
0.00636 | - |
NADPH | for FprA with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
0.0065 | - |
NADH | for FprB with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
0.00666 | - |
NADPH | for FprA with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
0.007 | - |
NADH | for FprB in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
0.00751 | - |
NADPH | for FprA with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
0.00866 | - |
NADH | for FprA with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
0.01177 | - |
NADPH | for FprB with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
0.01238 | - |
NADH | for FprA with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
0.0129 | - |
NADH | for FprB with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
0.01319 | - |
NADPH | for FprB with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
0.01424 | - |
NADPH | for FprB with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
0.01544 | - |
NADPH | for FprB with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
0.01866 | - |
NADH | for FprA with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
0.02967 | - |
NADPH | for FprB in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
0.05625 | - |
NADH | for FprA in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
0.1076 | - |
NADH | for FprA in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
wild type and mutants | - |
Pseudomonas putida KT 2240 | - |
wild type and mutants | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.05 | - |
electron donor NADH, cofactor FAD | Pseudomonas putida |
2.58 | - |
electron donor NADPH, cofactor FAD | Pseudomonas putida |
5.47 | - |
electron donor NADH, cofactor FMN | Pseudomonas putida |
5.73 | - |
electron donor NADPH, cofactor FMN | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fe(III) citrate + NADH | - |
Pseudomonas putida | Fe(II) citrate + NAD+ | - |
? | |
Fe(III) citrate + NADH | - |
Pseudomonas putida KT 2240 | Fe(II) citrate + NAD+ | - |
? | |
Fe(III) citrate + NADPH | - |
Pseudomonas putida | Fe(II) citrate + NADP+ | - |
? | |
Fe(III) citrate + NADPH | - |
Pseudomonas putida KT 2240 | Fe(II) citrate + NADP+ | - |
? | |
Fe(III)-EDTA + NADH | - |
Pseudomonas putida | reduced Fe(III)-EDTA + NAD+ | - |
? | |
Fe(III)-EDTA + NADH | - |
Pseudomonas putida KT 2240 | reduced Fe(III)-EDTA + NAD+ | - |
? | |
Fe(III)-EDTA + NADPH | - |
Pseudomonas putida | reduced Fe(III)-EDTA + NADP+ | - |
? | |
Fe(III)-EDTA + NADPH | - |
Pseudomonas putida KT 2240 | reduced Fe(III)-EDTA + NADP+ | - |
? | |
NADH + FAD | assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADH | Pseudomonas putida | NAD+ + reduced FAD | - |
? | |
NADH + FAD | assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADH | Pseudomonas putida KT 2240 | NAD+ + reduced FAD | - |
? | |
NADPH + FAD | assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADPH | Pseudomonas putida | NADP+ + reduced FAD | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ferredoxin-NADP+ reductase | - |
Pseudomonas putida |
FprA | Pseudomonas putida harbors two ferredoxin-NADP+ reductases FprA and FprB on its chromosome. | Pseudomonas putida |
FprB | Pseudomonas putida harbors two ferredoxin-NADP+ reductases FprA and FprB on its chromosome. | Pseudomonas putida |
Fprs | - |
Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.91 | - |
NADPH | for FprB in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
3.11 | - |
NADH | for FprA in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
5.6 | - |
NADH | for FprA in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
6.18 | - |
NADPH | for FprA in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
6.8 | - |
NADPH | for FprA with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
7.13 | - |
NADPH | for FprA in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
7.54 | - |
NADPH | for FprA with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
7.71 | - |
NADH | for FprA with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
8.21 | - |
NADH | for FprB in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
9.18 | - |
NADH | for FprA with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
10 | - |
NADH | for FprA with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
10.18 | - |
NADH | for FprA with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
10.28 | - |
NADPH | for FprB with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
10.64 | - |
NADH | for FprB in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
10.77 | - |
NADPH | for FprB with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
11.18 | - |
NADPH | for FprA with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
11.92 | - |
NADPH | for FprB in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
13.2 | - |
NADH | for FprB with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
16.44 | - |
NADH | for FprB with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
17.47 | - |
NADH | for FprB with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
19.24 | - |
NADH | for FprB with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
20.38 | - |
NADPH | for FprB with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
21.72 | - |
NADPH | for FprB with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
37.61 | - |
NADPH | for FprA with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | free FAD serves as an electron carrier | Pseudomonas putida | |
FMN | free FMN serves as an electron carrier | Pseudomonas putida |
General Information | Comment | Organism |
---|---|---|
metabolism | function in ferric iron metabolism | Pseudomonas putida |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
30 | - |
NADH | for FprA in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
90 | - |
NADPH | for FprB in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
110 | - |
NADH | for FprA in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
550 | - |
NADH | for FprA with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
720 | - |
NADPH | for FprB with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
770 | - |
NADPH | for FprB with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
840 | - |
NADPH | for FprA with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
930 | - |
NADH | for FprA with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
930 | - |
NADPH | for FprB with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1060 | - |
NADH | for FprA with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1060 | - |
NADH | for FprB with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1170 | - |
NADH | for FprB in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1190 | - |
NADPH | for FprA with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1320 | - |
NADPH | for FprA in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1570 | - |
NADPH | for FprA in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1680 | - |
NADPH | for FprA with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
2090 | - |
NADPH | for FprB in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
2690 | - |
NADH | for FprB with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
2870 | - |
NADH | for FprA with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
3130 | - |
NADH | for FprB with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
3270 | - |
NADH | for FprB in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
6040 | - |
NADPH | for FprA with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
7090 | - |
NADH | for FprB with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
17000 | - |
NADPH | for FprB with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida |