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Literature summary for 1.18.1.2 extracted from

  • Peregrina, J.R.; Herguedas, B.; Hermoso, J.A.; Martinez-Julvez, M.; Medina, M.
    Protein motifs involved in coenzyme interaction and enzymatic efficiency in anabaena ferredoxin-NADP+ reductase (2009), Biochemistry, 48, 3109-3119.
    View publication on PubMed
Search for more literature for 1.18.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed via plasmid in Escherichia coli Anabaena sp.

Protein Variants

Protein Variants Comment Organism
T155G/A160T/L263P called PP3, only half catalytic efficiency compared with wild type Anabaena sp.
T155G/A160T/L263P/R264P/G265P called PP5, increased Km but reduced kcat for NADPH Anabaena sp.
T155G/A160T/L263P/R264P/G265P/Y303S called PP5CT, no protein expressed Anabaena sp.
T155G/A160T/L263P/Y303S called PP3CT, catalytic efficiency comparable with wild type, high specificity for NADH Anabaena sp.
T155G/A160T/S223D/L263P/R264P/G265P called AMP1PP5, almost no reactivity Anabaena sp.
T155G/A160T/S223D/Y235F/L263P/R264P/G265P called AMP2PP5, almost no reactivity Anabaena sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0041
-
 NADPH mutant T155G/A160T/L263P/Y303S, called PP3CT Anabaena sp.
0.006
-
 NADPH wild type enzyme of Anabaena sp. Anabaena sp.
0.012
-
 NADPH mutant T155G/A160T/L263P, called PP3 Anabaena sp.
0.023
-
 NADPH mutant T155G/A160T/L263P/R264P/G265P, called PP5 Anabaena sp.
0.0394
-
 NADH mutant T155G/A160T/L263P/Y303S, called PP3CT Anabaena sp.
0.064
-
 NADH mutant T155G/A160T/L263P/R264P/G265P, called PP5 Anabaena sp.
0.39
-
 NADH mutant T155G/A160T/L263P, called PP3 Anabaena sp.
0.8
-
 NADH wild type enzyme of Anabaena sp. Anabaena sp.
1.25
-
 NADH mutant T155G/A160T/S223D/Y235F/L263P/R264P/G265P, called AMP2PP5 Anabaena sp.
1.323
-
 NADH mutant T155G/A160T/S223D/L263P/R264P/G265P, called AMP1PP5 Anabaena sp.
1.366
-
 NADPH mutant T155G/A160T/S223D/L263P/R264P/G265P, called AMP1PP5 Anabaena sp.

Organism

Organism UniProt Comment Textmining
Anabaena sp. P21890 PCC 7119
-

Purification (Commentary)

Purification (Comment) Organism
-
 Anabaena sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Anabaena sp. NADP+ + reduced 2,6-dichlorophenolindophenol
-
 r
oxidized 2,6-dichlorophenolindophenol + NADH
-
 Anabaena sp. NAD+ + reduced 2,6-dichlorophenolindophenol
-
 r

Synonyms

Synonyms Comment Organism
AnFNR FNR from Anabaena PCC 7119 Anabaena sp.
ferredoxin-NADP+ reductase
-
 Anabaena sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0003
-
 NADPH mutant T155G/A160T/S223D/L263P/R264P/G265P, called AMP1PP5 Anabaena sp.
0.0005
-
 NADH mutant T155G/A160T/S223D/Y235F/L263P/R264P/G265P, called AMP2PP5 Anabaena sp.
0.008
-
 NADH mutant T155G/A160T/S223D/L263P/R264P/G265P, called AMP1PP5 Anabaena sp.
0.16
-
 NADH wild type enzyme of Anabaena sp. Anabaena sp.
0.33
-
 NADH mutant T155G/A160T/L263P, called PP3 Anabaena sp.
0.65
-
 NADH mutant T155G/A160T/L263P/R264P/G265P, called PP5 Anabaena sp.
31
-
 NADH mutant T155G/A160T/L263P/Y303S, called PP3CT Anabaena sp.
35
-
 NADPH mutant T155G/A160T/L263P/R264P/G265P, called PP5 Anabaena sp.
38.5
-
 NADPH mutant T155G/A160T/L263P/Y303S, called PP3CT Anabaena sp.
77
-
 NADPH mutant T155G/A160T/L263P, called PP3 Anabaena sp.
81.5
-
 NADPH wild type enzyme of Anabaena sp. Anabaena sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0002
-
 NADPH mutant T155G/A160T/S223D/L263P/R264P/G265P, called AMP1PP5 Anabaena sp.
0.0004
-
 NADH mutant T155G/A160T/S223D/Y235F/L263P/R264P/G265P, called AMP2PP5 Anabaena sp.
0.006
-
 NADH mutant T155G/A160T/S223D/L263P/R264P/G265P, called AMP1PP5 Anabaena sp.
0.2
-
 NADH wild type enzyme of Anabaena sp. Anabaena sp.
0.8
-
 NADH mutant T155G/A160T/L263P, called PP3 Anabaena sp.
1.4
-
 NADH mutant T155G/A160T/L263P/R264P/G265P, called PP5 Anabaena sp.
800
-
 NADH mutant T155G/A160T/L263P/Y303S, called PP3CT Anabaena sp.
1500
-
 NADPH mutant T155G/A160T/L263P/R264P/G265P, called PP5 Anabaena sp.
6400
-
 NADPH mutant T155G/A160T/L263P, called PP3 Anabaena sp.
9400
-
 NADPH mutant T155G/A160T/L263P/Y303S, called PP3CT Anabaena sp.
13500
-
 NADPH wild type enzyme of Anabaena sp. Anabaena sp.