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Literature summary for 1.18.1.2 extracted from
- Effect of cadmium on ferredoxin:NADP+ oxidoreductase activity (2004), J. Inorg. Biochem., 98, 1338-1346.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | 70% inhibition, uncompetitive to dibromothymoquinone, noncompetitive inhibition of NADPH oxidation, Zn2+ diminishes the inhibitory effect for dibromothymoquinone reduction, but enhances inhibition of ferricyanide reduction, inhibitory effect on ferricyanide reduction, but on dibromothymoquinone reduction, is abolished by addition of 2-mercaptoethanol or histidine, inhibition mechnanism, overview | Spinacia oleracea |  |
| additional information | histidine and 2-mercaptoethanol are not inhibitory | Spinacia oleracea | |
| Zn2+ | 80% inhibition | Spinacia oleracea | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0031 | - |
NADPH | in presence of Cd2+ | Spinacia oleracea | |
| 0.0058 | - |
NADPH | - |
Spinacia oleracea | |
| 0.0119 | - |
dibromothymoquinone | - |
Spinacia oleracea | |
| 0.0132 | - |
dibromothymoquinone | in presence of Cd2+ | Spinacia oleracea | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Spinacia oleracea | 9507 | - |
| thylakoid membrane | tightly bound or loosely bound on the outer surface, 2 enzyme pools | Spinacia oleracea | 42651 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
native PAGE | Spinacia oleracea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| reduced ferredoxin + NADP+ | Spinacia oleracea | terminal step in the non-cyclic photosynthetic electron transfer chain | oxidized ferredoxin + NADPH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from spinach leaves in several steps, to homogeneity | Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricyanide + NADPH | i.e. K3Fe(CN)6, diaphorase activity | Spinacia oleracea | 2 ferrocyanide + NADP+ + H+ | - |
? | |
| dibromothymoquinone + NADPH | diaphorase activity | Spinacia oleracea | reduced dibromothymoquinone + NADP+ | - |
? | |
| reduced ferredoxin + NADP+ | - |
Spinacia oleracea | oxidized ferredoxin + NADPH | - |
? | |
| reduced ferredoxin + NADP+ | terminal step in the non-cyclic photosynthetic electron transfer chain | Spinacia oleracea | oxidized ferredoxin + NADPH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 35000, SDS-PAGE | Spinacia oleracea |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FNR | - |
Spinacia oleracea |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12.5 | - |
NADPH | in presence of Cd2+ | Spinacia oleracea | |
| 12.6 | - |
dibromothymoquinone | in presence of Cd2+ | Spinacia oleracea | |
| 23.2 | - |
NADPH | - |
Spinacia oleracea | |
| 25 | - |
dibromothymoquinone | - |
Spinacia oleracea | |
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Release 2023.1 (January 2023)
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