Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | 70% inhibition, uncompetitive to dibromothymoquinone, noncompetitive inhibition of NADPH oxidation, Zn2+ diminishes the inhibitory effect for dibromothymoquinone reduction, but enhances inhibition of ferricyanide reduction, inhibitory effect on ferricyanide reduction, but on dibromothymoquinone reduction, is abolished by addition of 2-mercaptoethanol or histidine, inhibition mechnanism, overview | Spinacia oleracea | |
additional information | histidine and 2-mercaptoethanol are not inhibitory | Spinacia oleracea | |
Zn2+ | 80% inhibition | Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0031 | - |
NADPH | in presence of Cd2+ | Spinacia oleracea | |
0.0058 | - |
NADPH | - |
Spinacia oleracea | |
0.0119 | - |
dibromothymoquinone | - |
Spinacia oleracea | |
0.0132 | - |
dibromothymoquinone | in presence of Cd2+ | Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Spinacia oleracea | 9507 | - |
thylakoid membrane | tightly bound or loosely bound on the outer surface, 2 enzyme pools | Spinacia oleracea | 42651 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
native PAGE | Spinacia oleracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced ferredoxin + NADP+ | Spinacia oleracea | terminal step in the non-cyclic photosynthetic electron transfer chain | oxidized ferredoxin + NADPH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
from spinach leaves in several steps, to homogeneity | Spinacia oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricyanide + NADPH | i.e. K3Fe(CN)6, diaphorase activity | Spinacia oleracea | 2 ferrocyanide + NADP+ + H+ | - |
? | |
dibromothymoquinone + NADPH | diaphorase activity | Spinacia oleracea | reduced dibromothymoquinone + NADP+ | - |
? | |
reduced ferredoxin + NADP+ | - |
Spinacia oleracea | oxidized ferredoxin + NADPH | - |
? | |
reduced ferredoxin + NADP+ | terminal step in the non-cyclic photosynthetic electron transfer chain | Spinacia oleracea | oxidized ferredoxin + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 35000, SDS-PAGE | Spinacia oleracea |
Synonyms | Comment | Organism |
---|---|---|
FNR | - |
Spinacia oleracea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.5 | - |
NADPH | in presence of Cd2+ | Spinacia oleracea | |
12.6 | - |
dibromothymoquinone | in presence of Cd2+ | Spinacia oleracea | |
23.2 | - |
NADPH | - |
Spinacia oleracea | |
25 | - |
dibromothymoquinone | - |
Spinacia oleracea |