Cloned (Comment) | Organism |
---|---|
gene fpr, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli | Rhodobacter capsulatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
NADPH | diaphorase activity, pH 8.0 | Rhodobacter capsulatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Rhodobacter capsulatus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
about, gel filtration | Rhodobacter capsulatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced ferredoxin + NADP+ | Rhodobacter capsulatus | - |
oxidized ferredoxin + NADPH | - |
? | |
reduced ferredoxin + NADP+ | Rhodobacter capsulatus 37b4 | - |
oxidized ferredoxin + NADPH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodobacter capsulatus | Q9L6V3 | - |
- |
Rhodobacter capsulatus 37b4 | Q9L6V3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli to homogeneity, native enzyme about 400fold to homogeneity | Rhodobacter capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl viologen + NADPH | diaphorase activity | Rhodobacter capsulatus | reduced methyl viologen + NADP+ | - |
? | |
methyl viologen + NADPH | diaphorase activity | Rhodobacter capsulatus 37b4 | reduced methyl viologen + NADP+ | - |
? | |
reduced ferredoxin + NADP+ | - |
Rhodobacter capsulatus | oxidized ferredoxin + NADPH | - |
? | |
reduced ferredoxin + NADP+ | - |
Rhodobacter capsulatus 37b4 | oxidized ferredoxin + NADPH | - |
? | |
reduced flavodoxin + NADP+ | - |
Rhodobacter capsulatus | oxidized flavodoxin + NADPH + H+ | - |
r | |
reduced flavodoxin + NADP+ | - |
Rhodobacter capsulatus 37b4 | oxidized flavodoxin + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30000, about, recombinant and native enzyme, SDS-PAGE | Rhodobacter capsulatus |
Synonyms | Comment | Organism |
---|---|---|
ferredoxin (flavodoxin)-(reduced) nicotinamide adenine dinucleotide phosphate reductase | - |
Rhodobacter capsulatus |
ferredoxin (flavodoxin)-NADP(H) reductase | - |
Rhodobacter capsulatus |
FPR | - |
Rhodobacter capsulatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
reduced flavodoxin | pH 8.0 | Rhodobacter capsulatus | |
7.2 | - |
NADPH | diaphorase activity, pH 8.0 | Rhodobacter capsulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Rhodobacter capsulatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | prosthetic group, tightly bound, can be released by heat denaturation | Rhodobacter capsulatus | |
NADP+ | - |
Rhodobacter capsulatus | |
NADPH | - |
Rhodobacter capsulatus |