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Literature summary for 1.18.1.2 extracted from

  • Wan, J.T.; Jarrett, J.T.
    Electron acceptor specificity of ferredoxin (flavodoxin):NADP+ oxidoreductase from Escherichia coli (2002), Arch. Biochem. Biophys., 406, 116-126.
    View publication on PubMed
Search for more literature for 1.18.1.2

Inhibitors

Inhibitors Comment Organism Structure
Ferredoxin inhibition of electron transfer at higher electron acceptor rate Escherichia coli
flavodoxinI/II inhibition of electron transfer at higher electron acceptor rate Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information thermodynamics and kinetics, measurement of direct electron transfer by stopped-flow spectrophotometry Escherichia coli
0.012
-
 Ferredoxin pH 8.0, 25°C Escherichia coli
0.012
-
 reduced ferredoxin pH 8.0, 25°C Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
FMNH2 + NADP+ Escherichia coli
-
 FMN + NADPH + H+
-
 ?
additional information Escherichia coli the enzyme reduces flavodoxin I and flavodoxin II, reaction of EC 1.19.1.1, and ferredoxin, ferredoxin being the kinetically and thermodynamically preferred partner. Ferredoxin binds to FNR with high affinity (Kd below 0.5 microM) and is reduced under single-turnover conditions. Flavodoxin I and flavodoxin II show affinities about 4- to 7fold weaker and reduction rates that are 10- to 100fold slower than those for ferredoxin ?
-
 ?
oxidized ferredoxin + NADPH Escherichia coli
-
 reduced ferredoxin + NADP+
-
 r
reduced ferredoxin + NADP+ Escherichia coli
-
 oxidized ferredoxin + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli P28861 enzyme catalyzes reactions of both EC 1.18.1.2 and EC 1.19.1.1
-

Reaction

Reaction Comment Organism Reaction ID
2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH reaction mechanism Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FMNH2 + NADP+
-
 Escherichia coli FMN + NADPH + H+
-
 ?
additional information specificity for tightly bound electron acceptors, overview Escherichia coli ?
-
 ?
additional information the enzyme reduces flavodoxin I and flavodoxin II, reaction of EC 1.19.1.1, and ferredoxin, ferredoxin being the kinetically and thermodynamically preferred partner. Ferredoxin binds to FNR with high affinity (Kd below 0.5 microM) and is reduced under single-turnover conditions. Flavodoxin I and flavodoxin II show affinities about 4- to 7fold weaker and reduction rates that are 10- to 100fold slower than those for ferredoxin Escherichia coli ?
-
 ?
oxidized ferredoxin + NADPH
-
 Escherichia coli reduced ferredoxin + NADP+
-
 r
oxidized ferredoxin + NADPH ferredoxin is the preferred electron acceptor Escherichia coli reduced ferredoxin + NADP+
-
 r
reduced ferredoxin + NADP+
-
 Escherichia coli oxidized ferredoxin + NADPH + H+
-
 r
reduced ferredoxin + NADP+
-
 Escherichia coli oxidized ferredoxin + NADPH + H+
-
 ?
reduced flavodoxin I + NADP+
-
 Escherichia coli oxidized flavodoxin I + NADPH + H+
-
 r
reduced flavodoxin II + NADP+
-
 Escherichia coli oxidized flavodoxin II + NADPH + H+
-
 r

Synonyms

Synonyms Comment Organism
ferredoxin (flavodoxin):NADP+ oxidoreductase
-
 Escherichia coli
FNR
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0039
-
 reduced flavodoxin II pH 8.0, 25°C Escherichia coli
0.0042
-
 reduced flavodoxin I pH 8.0, 25°C Escherichia coli
0.15
-
 reduced ferredoxin pH 8.0, 25°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at, steady-state kinetics Escherichia coli

Cofactor

Cofactor Comment Organism Structure
FAD involved in electron transfer in the reaction Escherichia coli
NADP+
-
 Escherichia coli
NADPH
-
 Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.05 0.1 Ferredoxin pH 8.0, 25°C Escherichia coli
0.05 0.1 flavodoxin I pH 8.0, 25°C Escherichia coli
0.05 0.1 flavodoxin II pH 8.0, 25°C Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
12.5
-
 reduced ferredoxin pH 8.0, 25°C Escherichia coli
19
-
 FMNH2 pH 8.0, 25°C Escherichia coli