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Literature summary for 1.18.1.1 extracted from
- Purification and characterization of an NADH-rubredoxin oxidoreductase involved in the utilization of oxygen by Desulfovibrio gigas (1993), Eur. J. Biochem., 216, 443-448.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Na2SO3 | - |
Megalodesulfovibrio gigas |  |
| NaCl | 70 mM, 50% inhibition | Megalodesulfovibrio gigas | |
| p-hydroxymercuribenzoate | - |
Megalodesulfovibrio gigas | |
| PCMB | - |
Megalodesulfovibrio gigas | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
x * 27000 + x * 32000, SDS-PAGE | Megalodesulfovibrio gigas |
| 32000 | - |
x * 27000 + x * 32000, SDS-PAGE | Megalodesulfovibrio gigas |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Megalodesulfovibrio gigas | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Megalodesulfovibrio gigas |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 12 | - |
- |
Megalodesulfovibrio gigas |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADH + oxidized rubredoxin | very specific towards Desulfovibrio gigas rubredoxin | Megalodesulfovibrio gigas | NAD+ + reduced rubredoxin | - |
? | |
| reduced Desulfovibrio gigas rubredoxin + NAD+ + H+ | - |
Megalodesulfovibrio gigas | oxidized Desulfovibrio gigas rubredoxin + NADH | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 27000 + x * 32000, SDS-PAGE | Megalodesulfovibrio gigas |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.42 | - |
Desulfovibrio gigas rubredoxin | - |
Megalodesulfovibrio gigas |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | prosthetic group | Megalodesulfovibrio gigas | |
| FMN | prosthetic group | Megalodesulfovibrio gigas | |
| NADH | - |
Megalodesulfovibrio gigas | |
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