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Literature summary for 1.17.99.4 extracted from

  • Hayaishi, O.; Kornberg, A.
    Metabolism of cytosine, thymine, uracil, and barbituric acid by bacterial enzymes (1952), J. Biol. Chem., 197, 717-732.
    View publication on PubMed
Search for more literature for 1.17.99.4

Activating Compound

Activating Compound Comment Organism Structure
methylene blue required as electron acceptor Corynebacterium sp.
methylene blue required as electron acceptor Mycobacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
 thymine
-
 Mycobacterium sp.
0.131
-
 Uracil
-
 Mycobacterium sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Corynebacterium sp. 5737
-
cytoplasm
-
 Mycobacterium sp. 5737
-

Organism

Organism UniProt Comment Textmining
Corynebacterium sp.
-
-
-
Corynebacterium sp. 161
-
-
-
Mycobacterium sp.
-
-
-

Storage Stability

Storage Stability Organism
-10°C, most stable at pH 9.0, no appreciable loss of activity for several months Mycobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
thymine + acceptor
-
 Corynebacterium sp. 5-methylbarbiturate + reduced acceptor
-
 ?
thymine + acceptor
-
 Mycobacterium sp. 5-methylbarbiturate + reduced acceptor
-
 ?
thymine + acceptor
-
 Corynebacterium sp. 161 5-methylbarbiturate + reduced acceptor
-
 ?
uracil + acceptor barbiturate, isobarbiturate, 5-methylbarbiturate, 6-methyluracil, dihydrothymine, dihydrouracil, 2-thiouracil, 2-thiothymine, 2-thio-5-methyluracil and cytosine are no substrates Corynebacterium sp. barbiturate + reduced acceptor
-
 ?
uracil + acceptor barbiturate, isobarbiturate, 5-methylbarbiturate, 6-methyluracil, dihydrothymine, dihydrouracil, 2-thiouracil, 2-thiothymine, 2-thio-5-methyluracil and cytosine are no substrates Mycobacterium sp. barbiturate + reduced acceptor
-
 ?
uracil + acceptor barbiturate, isobarbiturate, 5-methylbarbiturate, 6-methyluracil, dihydrothymine, dihydrouracil, 2-thiouracil, 2-thiothymine, 2-thio-5-methyluracil and cytosine are no substrates Corynebacterium sp. 161 barbiturate + reduced acceptor
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22 25 assay at Corynebacterium sp.
22 25 assay at Mycobacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Mycobacterium sp.

pH Range

pH Minimum pH Maximum Comment Organism
8 10 about half-maximal activity at pH 8.0 and 10 with thymine as substrate Mycobacterium sp.
8 9.7 about half-maximal activity at pH 8.0 and 9.7 with uracil as substrate Mycobacterium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
 90% loss of activity Mycobacterium sp.
9
-
 most stable at -10°C, no appreciable loss of activity for several months Mycobacterium sp.

Cofactor

Cofactor Comment Organism Structure
additional information no oxidation in the presence of O2 without methylene blue as electron acceptor Corynebacterium sp.
additional information no oxidation in the presence of O2 without methylene blue as electron acceptor Mycobacterium sp.