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Literature summary for 1.17.99.3 extracted from

  • Gustafsson, J.
    Biosynthesis of cholic acid in rat liver. 24-Hydroxylation of 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoic acid (1975), J. Biol. Chem., 250, 8243-8247.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ATP required Rattus norvegicus
GTP stimulates to a lesser degree than ATP Rattus norvegicus
ITP stimulates to a lesser degree than ATP Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
additional information oxygen may be obligatory since there is almost complete inhibition when the reaction is performed in an atmosphere consisting of nitrogen Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Rattus norvegicus 5739
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oyl-CoA + H2O + acceptor conversion of the coenzyme A ester is lower than with the free acid as substrate Rattus norvegicus 3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestan-26-oyl-CoA + reduced acceptor
-
?
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoic acid + H2O + acceptor
-
Rattus norvegicus 3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoic acid + reduced acceptor
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 pH 6.5: about 30% of maximal activity, pH 8.5: about 60% of maximal activity Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
coenzyme A marked inhibition of reaction with 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoic acid Rattus norvegicus
FMN slight Rattus norvegicus