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Literature summary for 1.17.99.3 extracted from

  • van Veldhoven, P.P.; van Rompuy, P.; Vanhooren, J.C.; Mannaerts, G.P.
    Purification and further characterization of peroxisomal trihydroxycoprostanoyl-CoA oxidase from rat liver (1994), Biochem. J., 304, 195-200.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
1,2-Butanediol activates Rattus norvegicus
1,2-propanediol activates Rattus norvegicus
1,3-Propanediol activates Rattus norvegicus
2,3-Butanediol activates Rattus norvegicus
albumin required Rattus norvegicus

General Stability

General Stability Organism
in presence of dithiothreitol, the enzyme is very stable in high concentrations of ethylene glycol,1,2-propanediol, 1,3-propanediol, 1,2-butanediol, 2,3-butanediol and 1,3-butanediol, 40-60 v/v Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
Rattus norvegicus 5777
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68800
-
2 * 68800, SDS-PAGE Rattus norvegicus
120000
-
gel filtration Rattus norvegicus
175000
-
non-denaturing PAGE Rattus norvegicus

Organic Solvent Stability

Organic Solvent Comment Organism
1,2-butanediol 40-60 v/v, in presence of dithiothreitol, the enzyme is very stable Rattus norvegicus
1,2-propanediol 40-60 v/v, in presence of dithiothreitol, the enzyme is very stable Rattus norvegicus
1,3-butanediol 40-60 v/v, in presence of dithiothreitol, the enzyme is very stable Rattus norvegicus
1,3-propanediol 40-60 v/v, in presence of dithiothreitol, the enzyme is very stable Rattus norvegicus
2,3-butanediol 40-60 v/v, in presence of dithiothreitol, the enzyme is very stable Rattus norvegicus
Ethylene glycol 40-60 v/v, in presence of dithiothreitol, the enzyme is very stable Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methylhexanoyl-CoA + H2O + acceptor
-
Rattus norvegicus 3-hydroxy-2-methylhexanoyl-CoA + reduced acceptor
-
?
2-methylpalmitoyl-CoA + H2O + acceptor
-
Rattus norvegicus 3-hydroxy-2-methylhexandecanoyl-CoA + reduced acceptor
-
?
pristanoyl-CoA + H2O + acceptor
-
Rattus norvegicus 3-hydroxy-1,6,10,14-tetramethylpentadecanoyl-CoA + reduced acceptor
-
?
trihydroxycoprostanoyl-CoA + H2O + acceptor
-
Rattus norvegicus 3alpha,7alpha,12alpha,24-tetrahydroxycoprostanoyl-CoA + reduced acceptor
-
?

Subunits

Subunits Comment Organism
dimer 2 * 68800, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
THCCox
-
Rattus norvegicus
Trihydroxycoprostanoyl-CoA oxidase
-
Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
FAD contains most likely 2 mol of loosely bound FAD per mol of enzyme Rattus norvegicus