Inhibitors | Comment | Organism | Structure |
---|---|---|---|
O2 | does not affect enzyme in cell extracts, inhibitory for purified enzyme | Azoarcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
ethylbenzene | - |
Azoarcus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Azoarcus sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
[Fe(III) (C5H5)2]+ | - |
Azoarcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
alpha,beta,gamma 1 * 96000 + 1 * 43000 + 1 * 23000, SDS-PAGE | Azoarcus sp. |
43000 | - |
alpha,beta,gamma 1 * 96000 + 1 * 43000 + 1 * 23000, SDS-PAGE | Azoarcus sp. |
96000 | - |
alpha,beta,gamma 1 * 96000 + 1 * 43000 + 1 * 23000, SDS-PAGE | Azoarcus sp. |
155000 | - |
gel filtration, native PAGE | Azoarcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethylbenzene + H2O + acceptor | Azoarcus sp. | anaerobic degradation of ethylbenzene in bacteria | (S)-1-phenylethanol + reduced acceptor | - |
ir | |
ethylbenzene + H2O + acceptor | Azoarcus sp. EbN1 | anaerobic degradation of ethylbenzene in bacteria | (S)-1-phenylethanol + reduced acceptor | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azoarcus sp. | - |
EbN1 | - |
Azoarcus sp. EbN1 | - |
EbN1 | - |
Purification (Comment) | Organism |
---|---|
- |
Azoarcus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0035 | - |
activity towards n-propylbenzene of cell extract from cells grown on ethylbenzene | Azoarcus sp. |
0.0224 | - |
activity towards ethylbenzene of cell extract from cells grown on ethylbenzene | Azoarcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethylbenzene + H2O + acceptor | anaerobic degradation of ethylbenzene in bacteria | Azoarcus sp. | (S)-1-phenylethanol + reduced acceptor | - |
ir | |
ethylbenzene + H2O + acceptor | anaerobic degradation of ethylbenzene in bacteria | Azoarcus sp. EbN1 | (S)-1-phenylethanol + reduced acceptor | - |
ir | |
ethylbenzene + H2O + [Fe(III) (C5H5)2]+ | - |
Azoarcus sp. | (S)-1-phenylethanol + [Fe(II) (C5H5)2] | - |
ir | |
ethylbenzene + H2O + [Fe(III) (C5H5)2]+ | - |
Azoarcus sp. EbN1 | (S)-1-phenylethanol + [Fe(II) (C5H5)2] | - |
ir | |
n-propylbenzene + H2O + [Fe(III) (C5H5)2]+ | - |
Azoarcus sp. | (S)-1-phenylpropanol + [Fe(II) (C5H5)2] | - |
? | |
n-propylbenzene + H2O + [Fe(III) (C5H5)2]+ | - |
Azoarcus sp. EbN1 | (S)-1-phenylpropanol + [Fe(II) (C5H5)2] | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | alpha,beta,gamma 1 * 96000 + 1 * 43000 + 1 * 23000, SDS-PAGE | Azoarcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Azoarcus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | about maximum activity at both pH | Azoarcus sp. |