Literature summary for 1.17.7.4 extracted from

Hao, G.; Shi, R.; Tao, R.; Fang, Q.; Jiang, X.; Ji, H.; Feng, L.; Huang, L.
Cloning, molecular characterization and functional analysis of 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase (HDR) gene for diterpenoid tanshinone biosynthesis in Salvia miltiorrhiza Bge. f. alba (2013), Plant Physiol. Biochem., 70, 21-32.

Cloned(Commentary)

Cloned (Comment)	Organism
gene HDR, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, functional complementation by SmHDR1 of the Escherichia coli HDR mutant MG1655 ara/ispH, overexpression of SmHDR1 accelerates the biosynthesis of beta-carotene and enhances the production of tanshinones in cultured hairy roots of Salvia miltiorrhiza Bge. f. alba, transformation using Agrobacterium rhizogenes strain ACCC10060	Salvia miltiorrhiza f. alba

Cloned (Comment)

Organism

gene HDR, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, functional complementation by SmHDR1 of the Escherichia coli HDR mutant MG1655 ara/ispH, overexpression of SmHDR1 accelerates the biosynthesis of beta-carotene and enhances the production of tanshinones in cultured hairy roots of Salvia miltiorrhiza Bge. f. alba, transformation using Agrobacterium rhizogenes strain ACCC10060

Salvia miltiorrhiza f. alba

Protein Variants

Protein Variants	Comment	Organism
additional information	recombinant overexpression of the enzyme leads to increased tanshinone content in SmHDR1 transgenic hairy roots, overview. The total levels of all three tanshinones are 0.70 to 1.04fold higher in the H lines than in the wild-type	Salvia miltiorrhiza f. alba

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast	the enzyme has a chloroplast transit peptide, the cleavage site of the putative N-terminal cTP of SmHDR1 is predicted to be located between Arg33 and Cys34, comprising 33 amino acid residues	Salvia miltiorrhiza f. alba	9507	-
additional information	no transmembrane regions in the SmHDR1 polypeptide	Salvia miltiorrhiza f. alba	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Iron-sulfur cluster	the iron-sulfur bridge is involved in catalysis	Salvia miltiorrhiza f. alba

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
51860	-	x * 51860, sequence calculation	Salvia miltiorrhiza f. alba

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+	Salvia miltiorrhiza f. alba	-	isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O	-	?
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+	Salvia miltiorrhiza f. alba	-	dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Salvia miltiorrhiza f. alba	T1RJ09	gene HDR	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
hairy root	-	Salvia miltiorrhiza f. alba	-
leaf	highest expression level	Salvia miltiorrhiza f. alba	-
root	-	Salvia miltiorrhiza f. alba	-
stem	-	Salvia miltiorrhiza f. alba	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+	-	Salvia miltiorrhiza f. alba	isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O	-	?
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+	-	Salvia miltiorrhiza f. alba	dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O	-	?

Subunits

Subunits	Comment	Organism
?	x * 51860, sequence calculation	Salvia miltiorrhiza f. alba

Synonyms

Synonyms	Comment	Organism
1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase	-	Salvia miltiorrhiza f. alba
HDR	-	Salvia miltiorrhiza f. alba

Cofactor

Cofactor	Comment	Organism	Structure
Ferredoxin	-	Salvia miltiorrhiza f. alba

pI Value

Organism	Comment	pI Value Maximum	pI Value
Salvia miltiorrhiza f. alba	sequence calculation	-	5.81

Expression

Organism	Comment	Expression
Salvia miltiorrhiza f. alba	expression of gene SmHDR1 is induced by 0.1 mM methyl-jasmonate and salicylic acid, but not by 0.1 mM abscisic acid, in hairy roots	up

General Information

General Information	Comment	Organism
physiological function	enzyme SmHDR1 is HDR is involved in the plastid nonmevalonate pathway of isoprenoid biosynthesis, it is an important enzyme involved in the biosynthesis of diterpenoid tanshinones in Salvia miltiorrhiza Bge. f. alba, HDR is key enzyme in the biosynthesis of precursors of isoprenoids	Salvia miltiorrhiza f. alba