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Literature summary for 1.17.7.4 extracted from

  • Kwon, M.; Shin, B.; Lee, J.; Han, J.; Kim, S.
    Characterization of Burkholderia glumae BGR1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (HDR), the terminal enzyme in 2-C-methyl-d-erythritol 4-phosphate (MEP) pathway (2013), J. Korean Soc. Appl. Biol. Chem., 56, 35-40.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Burkholderia glumae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate pH 8, temperature not specified in the publication Burkholderia glumae
0.0212
-
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate pH 8, temperature not specified in the publication Burkholderia glumae

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ iron content of BgHDR1 is determined as 1.6 Fe atoms per protein Burkholderia glumae
Fe2+ iron content of BgHDR1 is determined as 1.7 Fe atoms per protein Burkholderia glumae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49900
-
calculated from cDNA Burkholderia glumae
51330
-
calculated from cDNA Burkholderia glumae

Organism

Organism UniProt Comment Textmining
Burkholderia glumae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Burkholderia glumae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NADPH
-
Burkholderia glumae dimethylallyl diphosphate + isopentenyl diphosphate + NADP+ + H2O
-
?

Synonyms

Synonyms Comment Organism
(E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase
-
Burkholderia glumae
BgHDR1
-
Burkholderia glumae
BgHDR2
-
Burkholderia glumae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11
-
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate pH 8, temperature not specified in the publication Burkholderia glumae
3.11
-
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate pH 8, temperature not specified in the publication Burkholderia glumae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Burkholderia glumae

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Burkholderia glumae

General Information

General Information Comment Organism
malfunction HDR1 is able to complement an Escherichia coli hdr deletion mutant (DYTL1) Burkholderia glumae
malfunction HDR2 is able to complement an Escherichia coli hdr deletion mutant (DYTL1) Burkholderia glumae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
52.35
-
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate pH 8, temperature not specified in the publication Burkholderia glumae
518.3
-
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate pH 8, temperature not specified in the publication Burkholderia glumae