Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Burkholderia glumae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate | pH 8, temperature not specified in the publication | Burkholderia glumae | |
0.0212 | - |
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate | pH 8, temperature not specified in the publication | Burkholderia glumae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | iron content of BgHDR1 is determined as 1.6 Fe atoms per protein | Burkholderia glumae | |
Fe2+ | iron content of BgHDR1 is determined as 1.7 Fe atoms per protein | Burkholderia glumae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49900 | - |
calculated from cDNA | Burkholderia glumae |
51330 | - |
calculated from cDNA | Burkholderia glumae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia glumae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Burkholderia glumae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NADPH | - |
Burkholderia glumae | dimethylallyl diphosphate + isopentenyl diphosphate + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
(E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase | - |
Burkholderia glumae |
BgHDR1 | - |
Burkholderia glumae |
BgHDR2 | - |
Burkholderia glumae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.11 | - |
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate | pH 8, temperature not specified in the publication | Burkholderia glumae | |
3.11 | - |
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate | pH 8, temperature not specified in the publication | Burkholderia glumae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Burkholderia glumae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Burkholderia glumae |
General Information | Comment | Organism |
---|---|---|
malfunction | HDR1 is able to complement an Escherichia coli hdr deletion mutant (DYTL1) | Burkholderia glumae |
malfunction | HDR2 is able to complement an Escherichia coli hdr deletion mutant (DYTL1) | Burkholderia glumae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
52.35 | - |
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate | pH 8, temperature not specified in the publication | Burkholderia glumae | |
518.3 | - |
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate | pH 8, temperature not specified in the publication | Burkholderia glumae |