Cloned (Comment) | Organism |
---|---|
expressed as a Strep-tagged fusion protein in Escherichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
E126Q | inactive mutant used to trap a paramagnetic reaction intermediate representing the 3-CH2OH-rotated weak pi-complex, intermediate II | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purified by using Strep-tactin chromatography | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | results show a bioorganometallic mechanism of IspH catalysis. There are two paramagnetic reaction intermediates proposed in this mechanism. The intermediate trapped with IspH E126Q mutants represents the 3-CH2OH-rotated weak pi-complex, intermediate II. Following a formally two-electron reduction and dehydration of the substrate, intermediate II is converted into an eta3-allyl complex with an oxidized HiPIP-like cluster [4Fe-4S]3+, intermediate III, which is observed with wild type IspH | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
(E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase | - |
Escherichia coli |