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Literature summary for 1.17.4.4 extracted from

  • Rost, S.; Pelz, H.J.; Menzel, S.; MacNicoll, A.D.; Leon, V.; Song, K.J.; Jaekel, T.; Oldenburg, J.; Mueller, C.R.
    Novel mutations in the VKORC1 gene of wild rats and mice--a response to 50 years of selection pressure by warfarin? (2009), BMC Genet., 10, 4-4.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.17.4.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in HEK-293 cells Mus musculus

Protein Variants

Protein Variants Comment Organism
A143V mutant shows about 140% relative VKOR activity as compared to the wild type enzyme Rattus norvegicus
A26S mutant shows about 70% relative VKOR activity as compared to the wild type enzyme Mus musculus
A26T mutant shows about 60% relative VKOR activity as compared to the wild type enzyme Rattus norvegicus
A26T the mutation has only a moderate effect on VKOR activity with a reduction to approximately 56% of wild type activity Rattus norvegicus
A48T mutant shows about 120% relative VKOR activity as compared to the wild type enzyme Mus musculus
E37G mutant shows about 75% relative VKOR activity as compared to the wild type enzyme Mus musculus
E67K the mutant shows a reduced vitamin K epoxide turnover of about 33% compared to the wild type protein, and has no effect on warfarin sensitivity in vitro Rattus norvegicus
F63C the mutation reduces the VKOR activity to about 30% of normal Rattus norvegicus
I141V mutant shows about 45% relative VKOR activity as compared to the wild type enzyme Rattus norvegicus
I90L mutant shows about 90% relative VKOR activity as compared to the wild type enzyme Rattus norvegicus
L128Q mutant shows moderately reduced VKOR activity (about 60% compared to wild type protein) and is resistant to warfarin inhibition to a variable degree Rattus norvegicus
L128S mutant shows about 20% relative VKOR activity as compared to the wild type enzyme Mus musculus
R12W mutant shows about 35% relative VKOR activity as compared to the wild type enzyme Mus musculus
R33P VKOR activity of the Arg33Pro variant is reduced to 42% of wild type activity Rattus norvegicus
R58G mutant shows about 40% relative VKOR activity as compared to the wild type enzyme Mus musculus
R61L mutant shows about 50% relative VKOR activity as compared to the wild type enzyme Mus musculus
W59R mutant shows 16% residual VKOR activity Rattus norvegicus
Y139C mutant shows about 30% relative VKOR activity as compared to the wild type enzyme Mus musculus
Y139C mutant shows moderately reduced VKOR activity (about 60% compared to wild type protein) and is resistant to warfarin inhibition to a variable degree Rattus norvegicus
Y139S mutant shows moderately reduced VKOR activity and is resistant to warfarin inhibition to a variable degree Rattus norvegicus
Y39N mutant shows about 30% relative VKOR activity as compared to the wild type enzyme Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
warfarin almost complete inhibition at 0.06 mM Mus musculus
warfarin almost complete inhibition at 0.06 mM Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
vitamin K 2,3-epoxide + dithiothreitol
-
 Mus musculus vitamin K + oxidized dithiothreitol
-
 ?
vitamin K 2,3-epoxide + dithiothreitol
-
 Rattus norvegicus vitamin K + oxidized dithiothreitol
-
 ?

Synonyms

Synonyms Comment Organism
Vitamin K reductase
-
 Mus musculus
Vitamin K reductase
-
 Rattus norvegicus
vitamin K-epoxide reductase
-
 Mus musculus
vitamin K-epoxide reductase
-
 Rattus norvegicus
VKOR
-
 Mus musculus
VKOR
-
 Rattus norvegicus
VKORC1
-
 Mus musculus
VKORC1
-
 Rattus norvegicus