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Literature summary for 1.17.4.4 extracted from

  • Wajih, N.; Hutson, S.M.; Wallin, R.
    Disulfide dependent protein folding is linked to operation of the vitamin K cycle in the endoplasmic reticulum: A protein disulfide isomerase-VKORC1 redox enzyme complex appears to be responsible for vitamin K1 2,3-epoxide reduction (2006), J. Biol. Chem., 282, 2626-2635.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information bacitracin inhibition of reduced RNase triggers VKOR activity, overview Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
gene VKORC1, expression of the c-myc-tagged VKORC1 in HEK-293 cells and in BHK21 cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information siRNA silencing of VKOR complex subunit PDI Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum membrane
-
Rattus norvegicus 5789
-
microsome
-
Rattus norvegicus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,3-epoxy-2,3-dihydro-2-methyl-3-phytyl-1,4-naphthoquinone + 1,4-dithiothreitol Rattus norvegicus i.e. vitamin K 2,3-epoxide 2-hydroxy-2-methyl-3-phytyl-2,3-dihydronaphthoquinone + oxidized dithiothreitol i.e. vitamin K ?
additional information Rattus norvegicus the reduction is linked to dithiol-dependent oxidative folding of proteins in the ER by protein disulfide isomerase, PDI, oxidative folding of reduced RNase triggers the reduction of vitamin K epoxid and the gamma-carboxylation of the synthetic gamma-carboxylase peptide substrate FLEEL, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
gene VKORC1
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein VKORC1has a consensus sequence for glycosylation Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
native enzyme from liver microsomes by solubilization, pH 11, deoxycholate-Tris base microsome extraction procedure, immunoprecipitation, gel filtration, and protein A and protein G affinity chromatography Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-epoxy-2,3-dihydro-2-methyl-3-phytyl-1,4-naphthoquinone + 1,4-dithiothreitol i.e. vitamin K 2,3-epoxide Rattus norvegicus 2-hydroxy-2-methyl-3-phytyl-2,3-dihydronaphthoquinone + oxidized dithiothreitol i.e. vitamin K ?
additional information the reduction is linked to dithiol-dependent oxidative folding of proteins in the ER by protein disulfide isomerase, PDI, oxidative folding of reduced RNase triggers the reduction of vitamin K epoxid and the gamma-carboxylation of the synthetic gamma-carboxylase peptide substrate FLEEL, overview Rattus norvegicus ?
-
?

Subunits

Subunits Comment Organism
More the VKOR enzyme complex contains protein disulfide reductase, PDI, and VKORC1 as tightly associated subunits, complex organization and structure, overview, VKORC! is a highly hydrophobic protein Rattus norvegicus

Synonyms

Synonyms Comment Organism
vitamin K 2,3-epoxide reductase
-
Rattus norvegicus
VKOR
-
Rattus norvegicus