Inhibitors | Comment | Organism | Structure |
---|---|---|---|
warfarin | 0.0017 mM, 50% inhibition; mixed non-competitive inhibition vs. vitamin k 2,3-epoxide, competive inhibition vs. DTT | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00042 | - |
dithiothreitol | - |
Rattus norvegicus | |
0.0013 | - |
vitamin K 2,3-epoxide | - |
Rattus norvegicus | |
0.0016 | - |
vitamin K 2,3-epoxide | in the presence of 0.0017 mM R,S-warfarin | Rattus norvegicus | |
0.00212 | - |
dithiothreitol | in the presence of 0.0017 mM R,S-warfarin | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rattus norvegicus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
MonoS, hydroxyapatite, DEAE-Sepharose, Sephacryl S-200, partially purified enzyme | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000007 | - |
substrate vitamin K 2,3-epoxide, in the presence of 0.0017 mM R,S-warfarin | Rattus norvegicus |
0.000017 | - |
substrate vitamin K 2,3-epoxide | Rattus norvegicus |
0.00008 | - |
substrate dithiothreitol | Rattus norvegicus |
0.000137 | - |
- |
Rattus norvegicus |
0.00099 | - |
substrate dithiothreitol, in the presence of 0.0017 mM R,S-warfarin | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
vitamin K 2,3-epoxide + dithiothreitol | substrate vitamin K 2,3-epoxide, in the presence of 0.0017 mM R,S-warfarin | Rattus norvegicus | vitamin K + oxidized dithiothreitol | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | complex of at least two components, one possesing catalytic activity for the conversion of vitamin K 2,3-epoxide, second component contains the binding site for the exogenous (DTT) and endogenous disulphide reductant, this thiol-disulfide redox centre is also the site of warfarin binding | Rattus norvegicus |