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Literature summary for 1.17.4.2 extracted from

  • Holmgren, A.; Sengupta, R.
    The use of thiols by ribonucleotide reductase (2010), Free Radic. Biol. Med., 49, 1617-1628.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
caracemide
-
Escherichia coli
caracemide
-
Mus musculus
caracemide
-
Tequatrovirus T4
chlorambucil
-
Escherichia coli
chlorambucil
-
Mus musculus
chlorambucil
-
Tequatrovirus T4
cisplatin
-
Escherichia coli
cisplatin
-
Mus musculus
cisplatin
-
Tequatrovirus T4
Co2+ RNR activity chelates with copper leading to inactivation Escherichia coli
Co2+ RNR activity chelates with copper leading to inactivation Mus musculus
Co2+ RNR activity chelates with copper leading to inactivation Tequatrovirus T4

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ class II enzymes contain cobalamin as cofactor Mus musculus
Co2+ class II enzymes contain cobalamin as cofactor Escherichia coli
Fe2+ the class III enzymes contain 4Fe-4S clusters in the small beta-subunit Escherichia coli
Fe2+ the class III enzymes contain 4Fe-4S clusters in the small beta-subunit Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli class II RNRs convert nucleoside triphosphates and nucleoside 5'-diphosphates, see also EC 1.17.4.1, to the correspondent 2'-deoxy products, overview ?
-
?
additional information Mus musculus class II RNRs convert nucleoside triphosphates and nucleoside 5'-diphosphates, see also EC 1.17.4.1, to the correspondent 2'deoxy products, overview ?
-
?
nucleoside 5'-triphosphate + formate Escherichia coli class III RNR 2'-deoxynucleoside 5'-triphosphate + H2O
-
?
nucleoside 5'-triphosphate + formate Tequatrovirus T4 class III RNR 2'-deoxynucleoside 5'-triphosphate + H2O
-
?
nucleoside 5'-triphosphate + thioredoxin Mus musculus class II RNRs 2'-deoxynucleoside 5'-triphosphate + thioredoxin disulfide + H2O
-
?
nucleoside 5'-triphosphate + thioredoxin Escherichia coli class II RNRs 2'-deoxynucleoside 5'-triphosphate + thioredoxin disulfide + H2O
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Mus musculus
-
-
-
Tequatrovirus T4
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information class II RNRs convert nucleoside triphosphates and nucleoside 5'-diphosphates, see also EC 1.17.4.1, to the correspondent 2'-deoxy products, overview Escherichia coli ?
-
?
additional information class II RNRs convert nucleoside triphosphates and nucleoside 5'-diphosphates, see also EC 1.17.4.1, to the correspondent 2'deoxy products, overview Mus musculus ?
-
?
additional information the class II RNR also uses nucleoside 5'-diphosphate as substrate Mus musculus ?
-
?
additional information the class II RNR also uses nucleoside 5'-diphosphate as substrate Escherichia coli ?
-
?
nucleoside 5'-triphosphate + formate class III RNR Escherichia coli 2'-deoxynucleoside 5'-triphosphate + H2O
-
?
nucleoside 5'-triphosphate + formate class III RNR Tequatrovirus T4 2'-deoxynucleoside 5'-triphosphate + H2O
-
?
nucleoside 5'-triphosphate + thioredoxin class II RNRs Mus musculus 2'-deoxynucleoside 5'-triphosphate + thioredoxin disulfide + H2O
-
?
nucleoside 5'-triphosphate + thioredoxin class II RNRs Escherichia coli 2'-deoxynucleoside 5'-triphosphate + thioredoxin disulfide + H2O
-
?

Subunits

Subunits Comment Organism
monomer or dimer alpha or alpha2, class II RNRs Mus musculus
monomer or dimer alpha or alpha2, class II RNRs Escherichia coli
More structure comparisons of classI-III RNRs, model for the subunit organization of RNRs, overview Mus musculus
More structure comparisons of classI-III RNRs, model for the subunit organization of RNRs, overview Escherichia coli
More structure comparisons of classI-III RNRs, model for the subunit organization of RNRs, overview Tequatrovirus T4
tetramer alpha2beta2, class III RNRs Escherichia coli
tetramer alpha2beta2, class III RNRs Tequatrovirus T4

Synonyms

Synonyms Comment Organism
class II RNR cf. EC 1.17.4.1 Mus musculus
class II RNR cf. EC 1.17.4.1 Escherichia coli
class III RNR
-
Escherichia coli
class III RNR
-
Tequatrovirus T4

Cofactor

Cofactor Comment Organism Structure
5'-deoxyadenosylcobalamin class II enzymes Mus musculus
5'-deoxyadenosylcobalamin class II enzymes Escherichia coli
Cobalamin class II enzymes Mus musculus
Cobalamin class II enzymes Escherichia coli
additional information cofactor specificity and binding, role in reaction, overview Mus musculus
additional information cofactor specificity and binding, role in reaction, overview Escherichia coli
additional information the class III enzymes contain 4Fe-4S clusters in the small beta-subunit. Cofactor specificity and binding, role in reaction, overview Escherichia coli
additional information the class III enzymes contain 4Fe-4S clusters in the small beta-subunit. Cofactor specificity and binding, role in reaction, overview Tequatrovirus T4
S-adenosyl-L-methionine
-
Escherichia coli
S-adenosyl-L-methionine
-
Tequatrovirus T4
thioredoxin
-
Escherichia coli
thioredoxin class II enzymes Mus musculus

General Information

General Information Comment Organism
additional information Cys290 in class III from bacteriophage T4 are the conserved cysteine residues that are the sites for generation of the thiyl radical Tequatrovirus T4
additional information the class II RNR reaction involves deoxyadenosyl or cysteinyl radicals and is independent of oxygen. The thiyl radical in class II RNR is believed to be generated directly at the active site using the cofactor 5'-deoxyadenosylcobalamin Mus musculus
additional information the class II RNR reaction involves deoxyadenosyl or cysteinyl radicals and is independent of oxygen. The thiyl radical in class II RNR is believed to be generated directly at the active site using the cofactor 5'-deoxyadenosylcobalamin Escherichia coli
additional information the class III RNR reaction involves deoxyadenosyl, glycyl, or cysteinyl radicals and requires anaerobic conditions Escherichia coli
physiological function during anaerobic growth, Escherichia coli depends on a class III RNR for the synthesis of deoxyribonucleotides Escherichia coli