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Literature summary for 1.17.4.2 extracted from

  • Lohman, G.J.; Gerfen, G.J.; Stubbe, J.
    Inactivation of Lactobacillus leichmannii ribonucleotide reductase by 2,2-difluoro-2-deoxycytidine 5-triphosphate: adenosylcobalamin destruction and formation of a nucleotide-based radical (2010), Biochemistry, 49, 1396-1403.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.17.4.2

Inhibitors

Inhibitors Comment Organism Structure
2',2'-difluoro-2'-deoxycytidine 5'-triphosphate complete inactivation of the enzyme by 1 equiv of 2',2'-difluoro-2'-deoxyCTP, F2CTP, in about 2 min proceeding via alkylation by the sugar of F2CTP and AdoCbl destruction. 0.47 equiv of a sugar moiety is covalently bound to RNR and 0.25 equiv of a cobalt(III) corrin is tightly associated, likely through a covalent interaction with C419 (Co-S) in the active site of RNR. Analysis of the relationship of the nonalkylative pathway for RNR inactivation relative to the alkylative pathway, overview Lactobacillus leichmannii

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ enzyme requires adenosylcobalamin as a cofactor, the Co ion is involved in the reaction Lactobacillus leichmannii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Lactobacillus leichmannii RTPR catalyzes the conversion of nucleoside triphosphates to deoxynucleotides requiring adenosylcobalamin as a cofactor, model of reaction mechanism, overview ?
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Organism

Organism UniProt Comment Textmining
Lactobacillus leichmannii
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-
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dATP + adenosylcobalamin
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 Lactobacillus leichmannii ?
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 ?
additional information RTPR catalyzes the conversion of nucleoside triphosphates to deoxynucleotides requiring adenosylcobalamin as a cofactor, model of reaction mechanism, overview Lactobacillus leichmannii ?
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 ?

Synonyms

Synonyms Comment Organism
class II RNR
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 Lactobacillus leichmannii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
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 assay at Lactobacillus leichmannii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Lactobacillus leichmannii

Cofactor

Cofactor Comment Organism Structure
adenosylcobalamin
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 Lactobacillus leichmannii