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Literature summary for 1.17.4.2 extracted from

  • Jordan, A.; Torrents, E.; Jeanthon, C.; Eliasson, R.; Hellman, U.; Wernstedt, C.; Barbe, J.; Gibert, I.; Reichard, P.
    B12-dependent ribonucleotide reductases from deeply rooted eubacteria are structurally related to the aerobic enzyme from Escherichia coli (1997), Proc. Natl. Acad. Sci. USA, 94, 13487-13492.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
ATP
-
Deinococcus radiodurans
ATP stimulates CDP reduction Thermotoga maritima
dATP
-
Thermotoga maritima
dATP
-
Lactobacillus leichmannii
dATP
-
Deinococcus radiodurans

Cloned(Commentary)

Cloned (Comment) Organism
cloned by PCR from peptide sequence information Deinococcus radiodurans
cloned by PCR from peptide sequence information, nrdj gene sequenced completely and expressed in Escherichia coli BL21(DE3) Thermotoga maritima

Inhibitors

Inhibitors Comment Organism Structure
dGTP inhibits CDP reduction Deinococcus radiodurans
dGTP inhibits CDP reduction Thermotoga maritima
dTTP inhibits CDP reduction Deinococcus radiodurans
dTTP
-
Lactobacillus leichmannii
dTTP inhibits CDP reduction Thermotoga maritima

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Thermotoga maritima
Ca2+
-
Deinococcus radiodurans
Mg2+
-
Escherichia coli
Mg2+
-
Thermotoga maritima
Mg2+
-
Deinococcus radiodurans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
-
Pyrococcus furiosus
90000
-
gel filtraton Thermotoga maritima
107000
-
deduced amino acid sequence Deinococcus radiodurans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ribonucleoside triphosphate + reduced thioredoxin + H2O Escherichia coli
-
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O Pyrococcus furiosus
-
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O Thermoplasma acidophilum
-
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O Mycobacterium tuberculosis
-
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O Methanocaldococcus jannaschii
-
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O Archaeoglobus fulgidus
-
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O Thermotoga maritima
-
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O Lactobacillus leichmannii
-
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O Chloroflexus aurantiacus
-
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O Deinococcus radiodurans
-
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539
-
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus
-
-
-
Chloroflexus aurantiacus
-
J-10-fl (ATCC 29366)
-
Deinococcus radiodurans
-
-
-
Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539
-
-
-
Escherichia coli
-
-
-
Lactobacillus leichmannii
-
-
-
Methanocaldococcus jannaschii
-
-
-
Mycobacterium tuberculosis
-
-
-
Pyrococcus furiosus
-
-
-
Thermoplasma acidophilum
-
-
-
Thermotoga maritima
-
-
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermotoga maritima
-
Deinococcus radiodurans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CDP + reduced thioredoxin
-
Escherichia coli dCDP + oxidized thioredoxin + H2O
-
?
CDP + reduced thioredoxin
-
Pyrococcus furiosus dCDP + oxidized thioredoxin + H2O
-
?
CDP + reduced thioredoxin
-
Thermotoga maritima dCDP + oxidized thioredoxin + H2O
-
?
CDP + reduced thioredoxin
-
Lactobacillus leichmannii dCDP + oxidized thioredoxin + H2O
-
?
CDP + reduced thioredoxin
-
Deinococcus radiodurans dCDP + oxidized thioredoxin + H2O
-
?
CDP + reduced thioredoxin
-
Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 dCDP + oxidized thioredoxin + H2O
-
?
CDP + reduced thioredoxin
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 dCDP + oxidized thioredoxin + H2O
-
?
CTP + reduced thioredoxin
-
Thermotoga maritima dCTP + oxidized thioredoxin + H2O
-
?
CTP + reduced thioredoxin
-
Lactobacillus leichmannii dCTP + oxidized thioredoxin + H2O
-
?
CTP + reduced thioredoxin
-
Deinococcus radiodurans dCTP + oxidized thioredoxin + H2O
-
?
CTP + reduced thioredoxin
-
Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 dCTP + oxidized thioredoxin + H2O
-
?
CTP + reduced thioredoxin
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 dCTP + oxidized thioredoxin + H2O
-
?
ribonucleoside triphosphate + reduced thioredoxin
-
Escherichia coli 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin
-
Pyrococcus furiosus 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin
-
Thermoplasma acidophilum 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin
-
Mycobacterium tuberculosis 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin
-
Methanocaldococcus jannaschii 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin
-
Archaeoglobus fulgidus 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin
-
Thermotoga maritima 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin
-
Lactobacillus leichmannii 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin
-
Chloroflexus aurantiacus 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin
-
Deinococcus radiodurans 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin
-
Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O
-
Escherichia coli 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O
-
Pyrococcus furiosus 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O
-
Thermoplasma acidophilum 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O
-
Mycobacterium tuberculosis 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O
-
Methanocaldococcus jannaschii 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O
-
Archaeoglobus fulgidus 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O
-
Thermotoga maritima 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O
-
Lactobacillus leichmannii 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O
-
Chloroflexus aurantiacus 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O
-
Deinococcus radiodurans 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O
-
Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r
ribonucleoside triphosphate + reduced thioredoxin + H2O
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80 90
-
Thermotoga maritima

Cofactor

Cofactor Comment Organism Structure
coenzyme B12
-
Thermoplasma acidophilum
coenzyme B12
-
Mycobacterium tuberculosis
coenzyme B12 adenosylcobalamin Pyrococcus furiosus
coenzyme B12 adenosylcobalamin Lactobacillus leichmannii