Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dATP | specific activator for CTP reduction | Kitasatospora aureofaciens | |
dGTP | specific activator for ATP reduction | Kitasatospora aureofaciens |
General Stability | Organism |
---|---|
immobilization stabilizes the enzyme substantially, compared with free enzyme, which has an inactivation half-life of less than 1 day, on immobilization the inactivation half-life is about 15 days | Kitasatospora aureofaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
CTP | immobilized enzyme | Kitasatospora aureofaciens | |
0.01 | - |
UTP | immobilized enzyme | Kitasatospora aureofaciens | |
0.015 | - |
ATP | immobilized enzyme | Kitasatospora aureofaciens | |
0.07 | - |
ATP | in presence of effector dGTP | Kitasatospora aureofaciens | |
0.08 | - |
ADP | in presence of effector dGTP | Kitasatospora aureofaciens | |
0.085 | - |
CTP | presence of effector dATP | Kitasatospora aureofaciens | |
0.09 | - |
GTP | immobilized enzyme | Kitasatospora aureofaciens | |
0.11 | - |
CDP | presence of effector dATP | Kitasatospora aureofaciens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | markedly stimulates activity | Kitasatospora aureofaciens | |
Mg2+ | markedly stimulates activity | Kitasatospora aureofaciens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ribonucleoside triphosphate + reduced thioredoxin + H2O | Kitasatospora aureofaciens | key enzyme in the pathway of DNA biosynthesis in all organisms | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kitasatospora aureofaciens | - |
industrial strain BMK | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Kitasatospora aureofaciens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + reduced thioredoxin | - |
Kitasatospora aureofaciens | dADP + oxidized thioredoxin + H2O | - |
? | |
ATP + reduced thioredoxin | - |
Kitasatospora aureofaciens | dATP + oxidized thioredoxin + H2O | - |
? | |
CDP + reduced dithiothreitol | - |
Kitasatospora aureofaciens | dCDP + oxidized dithiothreitol + H2O | - |
r | |
CTP + reduced thioredoxin | - |
Kitasatospora aureofaciens | dCTP + oxidized thioredoxin + H2O | - |
? | |
GTP + reduced thioredoxin | - |
Kitasatospora aureofaciens | dGTP + oxidized thioredoxin + H2O | - |
? | |
ribonucleoside triphosphate + dithioerythritol | - |
Kitasatospora aureofaciens | 2'-deoxyribonucleoside triphosphate + ? + H2O | - |
? | |
ribonucleoside triphosphate + reduced thioredoxin | - |
Kitasatospora aureofaciens | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin + H2O | key enzyme in the pathway of DNA biosynthesis in all organisms | Kitasatospora aureofaciens | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
UTP + reduced thioredoxin | - |
Kitasatospora aureofaciens | dUTP + oxidized thioredoxin + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Kitasatospora aureofaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Kitasatospora aureofaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Kitasatospora aureofaciens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.4 | 9.6 | - |
Kitasatospora aureofaciens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
coenzyme B12 | - |
Kitasatospora aureofaciens |