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Literature summary for 1.17.4.2 extracted from
- Substrate and effector binding to ribonucleoside triphosphate reductase of Lactobacillus leichmannii (1974), Biochemistry, 13, 654-661.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dATP | stimulation of CTP reduction | Lactobacillus leichmannii |  |
| dCTP | stimulation of UTP reduction | Lactobacillus leichmannii | |
| dGTP | stimulation of ATP reduction | Lactobacillus leichmannii | |
| dTTP | stimulation of GTP reduction | Lactobacillus leichmannii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 69300 | - |
enzyme in 6 M guanidine-HCl, 0.1 M dithiothreitol, equilibrium ultracentrifugation | Lactobacillus leichmannii |
| 72500 | - |
native enzyme in 0.1 M glycine buffer, pH 9.1, equilibrium ultracentrifugation | Lactobacillus leichmannii |
| 72600 | - |
maleated enzyme in 0.05 M phosphate buffer, equilibrium ultracentrifugation | Lactobacillus leichmannii |
| 74500 | - |
enzyme in 6 M guanidine-HCl, 0.1 M dithiothreitol, heated for 20 min at 60°C, equilibrium ultracentrifugation | Lactobacillus leichmannii |
| 75300 | - |
native enzyme in 0.05 M phosphate buffer, pH 7.0, sedimentation equilibrium method | Lactobacillus leichmannii |
| 76000 | - |
gel filtration | Lactobacillus leichmannii |
| 78400 | - |
SDS-PAGE | Lactobacillus leichmannii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | Lactobacillus leichmannii | - |
ribonucleoside triphosphate + reduced thioredoxin | - |
r | |
| ribonucleoside triphosphate + reduced thioredoxin + H2O | Lactobacillus leichmannii | - |
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactobacillus leichmannii | - |
strain ATCC 7630 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lactobacillus leichmannii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 16 | - |
- |
Lactobacillus leichmannii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -65°C, no detectable loss of enzyme has been observed during several years of storage of packed bacteria | Lactobacillus leichmannii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
Lactobacillus leichmannii | ribonucleoside triphosphate + reduced thioredoxin | - |
r | |
| ribonucleoside triphosphate + reduced thioredoxin | - |
Lactobacillus leichmannii | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
| ribonucleoside triphosphate + reduced thioredoxin + H2O | - |
Lactobacillus leichmannii | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 78400, SDS-PAGE | Lactobacillus leichmannii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| coenzyme B12 | - |
Lactobacillus leichmannii | |
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