Activating Compound | Comment | Organism | Structure |
---|---|---|---|
5'-deoxy-5'-adenosylcobalamin | - |
Thermus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
GTP | at 37°C | Thermus sp. | |
0.32 | - |
CTP | at 37°C | Thermus sp. | |
0.99 | - |
GTP | at 70°C | Thermus sp. | |
1 | - |
ATP | at 70°C | Thermus sp. | |
1.4 | - |
CTP | at 70°C | Thermus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | stimulates enzyme activity | Thermus sp. | |
Mn2+ | stimulates enzyme activity | Thermus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
gel filtration | Thermus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ribonucleoside triphosphate + reduced thioredoxin + H2O | Thermus aquaticus | - |
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin + H2O | Thermus sp. | - |
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin + H2O | Lactobacillus leichmannii | - |
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin + H2O | Thermus aquaticus YT-1 | - |
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus leichmannii | - |
- |
- |
Thermus aquaticus | - |
- |
- |
Thermus aquaticus YT-1 | - |
- |
- |
Thermus sp. | - |
X-1 | - |
Thermus sp. X-1 | - |
X-1 | - |
Purification (Comment) | Organism |
---|---|
- |
Lactobacillus leichmannii |
partially | Thermus sp. |
Storage Stability | Organism |
---|---|
20°C, 0.03 M dimethylglutarate buffer, pH 7.2, remains fully active on storage for 24 h | Thermus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + reduced thioredoxin | - |
Thermus sp. | dADP + oxidized thioredoxin + H2O | - |
? | |
ADP + reduced thioredoxin | - |
Lactobacillus leichmannii | dADP + oxidized thioredoxin + H2O | - |
? | |
ATP + reduced thioredoxin | - |
Thermus sp. | dATP + oxidized thioredoxin + H2O | - |
? | |
ATP + reduced thioredoxin | - |
Lactobacillus leichmannii | dATP + oxidized thioredoxin + H2O | - |
? | |
ATP + reduced thioredoxin | - |
Thermus sp. X-1 | dATP + oxidized thioredoxin + H2O | - |
? | |
CDP + reduced thioredoxin | - |
Thermus sp. | dCDP + oxidized thioredoxin + H2O | - |
? | |
CDP + reduced thioredoxin | - |
Lactobacillus leichmannii | dCDP + oxidized thioredoxin + H2O | - |
? | |
CMP + reduced thioredoxin | - |
Thermus sp. | dCMP + oxidized thioredoxin + H2O | - |
? | |
CMP + reduced thioredoxin | - |
Lactobacillus leichmannii | dCMP + oxidized thioredoxin + H2O | - |
? | |
CTP + reduced thioredoxin | - |
Thermus sp. | dCTP + oxidized thioredoxin + H2O | - |
? | |
CTP + reduced thioredoxin | - |
Lactobacillus leichmannii | dCTP + oxidized thioredoxin + H2O | - |
? | |
CTP + reduced thioredoxin | - |
Thermus sp. X-1 | dCTP + oxidized thioredoxin + H2O | - |
? | |
GDP + reduced thioredoxin | - |
Thermus sp. | dGDP + oxidized thioredoxin + H2O | - |
? | |
GDP + reduced thioredoxin | - |
Lactobacillus leichmannii | dGDP + oxidized thioredoxin + H2O | - |
? | |
GTP + reduced thioredoxin | - |
Thermus sp. | dGTP + oxidized thioredoxin + H2O | - |
? | |
GTP + reduced thioredoxin | - |
Lactobacillus leichmannii | dGTP + oxidized thioredoxin + H2O | - |
? | |
GTP + reduced thioredoxin | - |
Thermus sp. X-1 | dGTP + oxidized thioredoxin + H2O | - |
? | |
IDP + reduced thioredoxin | - |
Thermus sp. | dIDP + oxidized thioredoxin | - |
? | |
ITP + reduced thioredoxin | - |
Thermus sp. | dITP + oxidized thioredoxin | - |
? | |
additional information | GMP and AMP are no substrates | Thermus sp. | ? | - |
? | |
additional information | GMP and AMP are no substrates | Thermus sp. X-1 | ? | - |
? | |
ribonucleoside triphosphate + reduced thioredoxin | - |
Thermus aquaticus | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin | - |
Thermus sp. | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin | - |
Lactobacillus leichmannii | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin | - |
Thermus sp. X-1 | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin | - |
Thermus aquaticus YT-1 | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin + H2O | - |
Thermus aquaticus | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin + H2O | - |
Thermus sp. | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin + H2O | - |
Lactobacillus leichmannii | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin + H2O | - |
Thermus aquaticus YT-1 | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
UDP + reduced thioredoxin | - |
Thermus sp. | dUDP + oxidized thioredoxin + H2O | - |
? | |
UDP + reduced thioredoxin | - |
Lactobacillus leichmannii | dUDP + oxidized thioredoxin + H2O | - |
? | |
UMP + reduced thioredoxin | - |
Thermus sp. | dUMP + oxidized thioredoxin + H2O | - |
? | |
UTP + reduced thioredoxin | - |
Thermus sp. | dUTP + oxidized thioredoxin + H2O | - |
? | |
UTP + reduced thioredoxin | - |
Lactobacillus leichmannii | dUTP + oxidized thioredoxin + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Lactobacillus leichmannii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Lactobacillus leichmannii |
70 | - |
- |
Thermus sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | - |
Lactobacillus leichmannii |
20 | 80 | - |
Thermus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8.4 | - |
Thermus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
coenzyme B12 | adenosylcobalamin | Lactobacillus leichmannii |