Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dATP | - |
Lactobacillus leichmannii | |
dGTP | specific activator for ATP reduction | Lactobacillus leichmannii | |
NADPH | stimulates reductase activity in crude extracts | Lactobacillus leichmannii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dTTP | inhibits ATP reduction | Lactobacillus leichmannii | |
Mg2+ | strongly inhibitory in absence of ATP | Lactobacillus leichmannii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
CTP | - |
Lactobacillus leichmannii | |
2.3 | - |
ATP | - |
Lactobacillus leichmannii | |
9.5 | - |
CTP | without ATP | Lactobacillus leichmannii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
sedimentation equilibrium analysis | Lactobacillus leichmannii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ribonucleoside triphosphate + reduced thioredoxin + H2O | Lactobacillus leichmannii | physiological hydrogen donor is unknown | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus leichmannii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Lactobacillus leichmannii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
576 | - |
- |
Lactobacillus leichmannii |
Storage Stability | Organism |
---|---|
-20°C, crude extracts relatively unstable, most of the activity disappears within a few weeks | Lactobacillus leichmannii |
0-4°C, acetone and hydroxylapatite fractions are quite stable, at least 70-80% of their activities remain after 3 months | Lactobacillus leichmannii |
0-4°C, crude extracts relatively unstable, most of the activity disappears within a few weeks | Lactobacillus leichmannii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + reduced thioredoxin | most active diphosphate substrate | Lactobacillus leichmannii | dADP + oxidized thioredoxin + H2O | - |
? | |
ATP + reduced thioredoxin | - |
Lactobacillus leichmannii | dATP + oxidized thioredoxin + H2O | - |
? | |
CDP + reduced thioredoxin | poorly reduced | Lactobacillus leichmannii | dCDP + oxidized thioredoxin + H2O | - |
? | |
CMP + reduced thioredoxin | poorly reduced | Lactobacillus leichmannii | dCMP + oxidized thioredoxin + H2O | - |
? | |
CTP + reduced thioredoxin | - |
Lactobacillus leichmannii | dCTP + oxidized thioredoxin + H2O | - |
? | |
GDP + reduced thioredoxin | poorly reduced | Lactobacillus leichmannii | dGDP + oxidized thioredoxin + H2O | - |
? | |
GTP + reduced thioredoxin | - |
Lactobacillus leichmannii | dGTP + oxidized thioredoxin + H2O | - |
? | |
ribonucleoside triphosphate + reduced thioredoxin | - |
Lactobacillus leichmannii | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin + H2O | physiological hydrogen donor is unknown | Lactobacillus leichmannii | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
UDP + reduced thioredoxin | poorly reduced | Lactobacillus leichmannii | dUDP + oxidized thioredoxin + H2O | - |
? | |
UTP + reduced thioredoxin | - |
Lactobacillus leichmannii | dUTP + oxidized thioredoxin + H2O | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,6-dimethylbenzimidazolycobamide | - |
Lactobacillus leichmannii | |
coenzyme B12 | - |
Lactobacillus leichmannii | |
dihydrolipoate | - |
Lactobacillus leichmannii |