Literature summary for 1.17.4.1 extracted from

Fairman, J.W.; Wijerathna, S.R.; Ahmad, M.F.; Xu, H.; Nakano, R.; Jha, S.; Prendergast, J.; Welin, R.M.; Flodin, S.; Roos, A.; Nordlund, P.; Li, Z.; Walz, T.; Dealwis, C.G.
Structural basis for allosteric regulation of human ribonucleotide reductase by nucleotide-induced oligomerization (2011), Nat. Struct. Mol. Biol., 18, 316-322.

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	TTP, dATP, TTP/GDP, TTP/ATP, and TTP/dATP, 1. TTP bound at the S-site, 2. dATP bound at the S-site, 3. TTP bound at the S-site and GDP at the C-site, 4. TTP bound at the S-site and ATP at the A-site, and 5. TTP bound at the S-site and dATP at the A-site	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
subunit RR1 in complex with TTP, dATP, TTP/GDP, TTP/ATP, and TTP/dATP, 1. TTP bound at the S-site, 2. dATP bound at the S-site, 3. TTP bound at the S-site and GDP at the C-site, 4. TTP bound at the S-site and ATP at the A-site, and 5. TTP bound at the S-site and dATP at the A-site, X-ray diffraction structure determination and analysis at resolutions of 2.4 A, 2.3 A, 3.2 A, 3.1 A, and 3.1 A, respectively	Homo sapiens

Crystallization (Comment)

Organism

subunit RR1 in complex with TTP, dATP, TTP/GDP, TTP/ATP, and TTP/dATP, 1. TTP bound at the S-site, 2. dATP bound at the S-site, 3. TTP bound at the S-site and GDP at the C-site, 4. TTP bound at the S-site and ATP at the A-site, and 5. TTP bound at the S-site and dATP at the A-site, X-ray diffraction structure determination and analysis at resolutions of 2.4 A, 2.3 A, 3.2 A, 3.1 A, and 3.1 A, respectively

Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
D16R	site-directed mutagenesis, the mutant retains 55% of wild-type activity for CDP reduction, and 67% for ADP reduction, it is not inhibited and does not form hexamers at physiologically relevant dATP concentrations	Homo sapiens
D57N	site-directed mutagenesis, the mutant is not inhibited and does not form hexamers at physiologically relevant dATP concentrations, the mutation of Asp 57 to Asn abolishes the salt-bridge and change the electrostatic environment of the A-site, resulting in loss of allosteric regulation by dATP	Homo sapiens
H2E	site-directed mutagenesis, the mutant retains 56% of wild-type activity for CDP reduction, and 56% for ADP reduction	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Subunits

Subunits	Comment	Organism
More	dATP-induced oligomerization, overview, modeling of the holo-complex	Homo sapiens
multimer	alphanbetan multi-subunit protein complex consisting of subunit types RR1 and RR2, the alpha or RR1 subunit contains the catalytic C site and two allosteric sites, while the beta or RR2 subunit houses a stable tyrosyl free radical that is transferred some 35 A to the catalytic site to initiate radical-based chemistry on the substrate	Homo sapiens

Synonyms

Synonyms	Comment	Organism
class I RR	-	Homo sapiens

General Information

General Information	Comment	Organism
additional information	RR is regulated transcriptionally and allosterically. RR activity is also controlled by subunit RR2 levels	Homo sapiens
additional information	structural basis for substrate selection, RR activity is lowest at high dATP concentrations when the hexamer population is high, egulation of RR by dATPinduced oligomerization, overview. RR is regulated transcriptionally and allosterically. RR is further regulated by subunit localization and by its protein inhibitor Sml1	Homo sapiens