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Literature summary for 1.17.4.1 extracted from

  • Fieschi, F.; Torrents, E.; Toulokhonova, L.; Jordan, A.; Hellman, U.; Barbe, J.; Gibert, I.; Karlsson, M.; Sjoberg, B.M.
    The manganese-containing ribonucleotide reductase of Corynebacterium ammoniagenes is a class Ib enzyme (1998), J. Biol. Chem., 273, 4329-4337.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ATP stimulation of CDP reduction Corynebacterium ammoniagenes
dATP stimulation of CDP reduction Corynebacterium ammoniagenes
dTTP stimulation of CDP reduction Corynebacterium ammoniagenes

Cloned(Commentary)

Cloned (Comment) Organism
expression R1E and R2F subunits in Escherichia coli Corynebacterium ammoniagenes

Inhibitors

Inhibitors Comment Organism Structure
Hydroxyurea 2 mM, approx. 80% inactivation Corynebacterium ammoniagenes

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ EPR-silent Mn bound to the polypeptide chain, approx. 0.5 mol manganese ions/mol of R2F polypeptide Corynebacterium ammoniagenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37900
-
alpha,beta2, 1 * 81200 + 2 * 37900, deduced from nucleotide sequence Corynebacterium ammoniagenes
81200
-
alpha,beta2, 1 * 81200 + 2 * 37900, deduced from nucleotide sequence Corynebacterium ammoniagenes
160000
-
gel filtration Corynebacterium ammoniagenes

Organism

Organism UniProt Comment Textmining
Corynebacterium ammoniagenes O69273 class Ib reductase
-

Purification (Commentary)

Purification (Comment) Organism
low-salt precipitation, DEAE-cellulose, Supredex 200, Mono Q Corynebacterium ammoniagenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.034
-
-
Corynebacterium ammoniagenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ribonucleoside diphosphate + reduced thioredoxin NrdH-redoxin obtained from an overproducing strain, no activity with E. coli thioredoxin Corynebacterium ammoniagenes 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
ir

Subunits

Subunits Comment Organism
trimer alpha,beta2, 1 * 81200 + 2 * 37900, deduced from nucleotide sequence Corynebacterium ammoniagenes