Literature summary for 1.17.4.1 extracted from

Jordan, A.; Pontis, E.; Atta, M.; Krook, M.; Gibert, I.; Barbe, J.; Reichard, P.
A second class I ribonucleotide reductase in Enterobacteriaceae: characterization of the Salmonella typhimurium enzyme (1994), Proc. Natl. Acad. Sci. USA, 91, 12892-12896.

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Activating Compound

Activating Compound	Comment	Organism	Structure
dATP	-	Escherichia coli
dATP	strong stimulation of CDP reduction	Salmonella enterica subsp. enterica serovar Typhimurium
dGTP	-	Escherichia coli
dGTP	slight stimulation of CDP reduction	Salmonella enterica subsp. enterica serovar Typhimurium
dTTP	-	Escherichia coli
dTTP	slight stimulation of CDP reduction	Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors	Comment	Organism	Structure
Hydroxyurea	-	Escherichia coli
Hydroxyurea	approx. 0.5 mM, 50% inhibition	Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00013	-	glutaredoxin	-	Escherichia coli
0.003	-	dATP	positive effector of CDP reduction	Salmonella enterica subsp. enterica serovar Typhimurium
0.006	-	glutaredoxin 1	-	Salmonella enterica subsp. enterica serovar Typhimurium
0.016	-	CDP	-	Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Iron	-	Escherichia coli
Iron	1.8 mol of iron per mol of R2F subunit, dinuclear iron center	Salmonella enterica subsp. enterica serovar Typhimurium
Mg2+	-	Escherichia coli
Mg2+	activity depends on Mg2+	Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
36000	-	alpha2,beta2, 2 * 70000 + 2 * 36000, SDS-PAGE	Salmonella enterica subsp. enterica serovar Typhimurium
70000	-	alpha2,beta2, 2 * 70000 + 2 * 36000, SDS-PAGE	Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-
Salmonella enterica subsp. enterica serovar Typhimurium	-	class I ribonucleotide reductase	-

Purification (Commentary)

Purification (Comment)	Organism
R1E subunit, ammonium sulfate, DEAE-Sepharose, dATP-Sepharose	Salmonella enterica subsp. enterica serovar Typhimurium
R2F subunit, ammonium sulfate, DEAE-Sepharose, Mono Q, Superdex	Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.28	-	CDP reduction of subunit R1E in the presence of subunit R2F	Salmonella enterica subsp. enterica serovar Typhimurium
0.83	-	CDP reduction of subunit R2F in the presence of subunit R1E	Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
CDP + reduced glutaredoxin 1	-	Salmonella enterica subsp. enterica serovar Typhimurium	dCDP + oxidized glutaredoxin 1 + H2O	-	r
CDP + thioredoxin	-	Salmonella enterica subsp. enterica serovar Typhimurium	2'-dCDP + thioredoxin disulfide + H2O	-	r
ribonucleoside diphosphate + reduced glutaredoxin	-	Escherichia coli	2'-deoxyribonucleoside diphosphate + oxidized glutaredoxin + H2O	-	ir
ribonucleoside diphosphate + reduced glutaredoxin 1	-	Salmonella enterica subsp. enterica serovar Typhimurium	2'-deoxyribonucleoside diphosphate + oxidized glutaredoxin 1 + H2O	-	ir
ribonucleoside diphosphate + reduced thioredoxin	-	Escherichia coli	2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O	-	ir
ribonucleoside diphosphate + reduced thioredoxin	dithiothreitol serves as in vitro electron donor, maximal activity with 40 mM	Salmonella enterica subsp. enterica serovar Typhimurium	2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O	-	ir

Subunits

Subunits	Comment	Organism
tetramer	-	Escherichia coli
tetramer	alpha2,beta2, 2 * 70000 + 2 * 36000, SDS-PAGE	Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Salmonella enterica subsp. enterica serovar Typhimurium

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Release 2023.1 (January 2023)