Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphate | 50 mM, slight stimulation, inhibition at 200 mM | Saccharomyces cerevisiae |
General Stability | Organism |
---|---|
partially purified enzyme is very unstable in solution, half-life at 0°C: less than 24 h | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | - |
Herpes simplex virus | |
additional information | not inhibited by 8-hydroxyquinoline and o-phenanthroline | Saccharomyces cerevisiae | |
YAGAVVNDL | peptide may prevent association of the two subunits by competing for the subunit binding site | Herpes simplex virus | |
YGAVVNDL | - |
Herpes simplex virus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
thioredoxin | - |
Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Herpes simplex virus | - |
type 1, HSV-1 | - |
Saccharomyces cerevisiae | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0072 | - |
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
quick-freezing in liquid nitrogen and subsequent storage at -20°C, several weeks, no loss of activity | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ribonucleoside diphosphate + reduced thioredoxin | - |
Herpes simplex virus | 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O | - |
ir | |
ribonucleoside diphosphate + reduced thioredoxin | maximal activity with E. coli thioredoxin | Saccharomyces cerevisiae | 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O | - |
ir |