Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | stimulation of UDP reduction | Escherichia coli | |
ATP | stimulation of CDP reduction | Escherichia coli | |
dATP | stimulation of CDP and UDP reduction | Escherichia coli | |
dGTP | stimulation of GDP reduction | Escherichia coli | |
dGTP | stimulation of ADP reduction | Escherichia coli | |
dTTP | stimulation of UDP reduction | Escherichia coli | |
dTTP | stimulation of CDP reduction | Escherichia coli | |
dTTP | stimulation of GDP reduction | Escherichia coli | |
dTTP | stimulation of ADP reduction | Escherichia coli | |
additional information | ribonucleoside effectors are exclusively bound at effector binding sites on subunit B1 controlling substrate specificity and activity | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
overproducing strain | - |
Escherichia coli overproducing | - |
overproducing strain | - |
Purification (Comment) | Organism |
---|---|
chimeric R2 genes in which C-terminal sequences in Escherichia coli subunit R2 are replaced by C-terminal sequences from the mouse subunit R2 | Escherichia coli |
overproducing strain | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.59 | - |
purified subunit B1 | Escherichia coli |
2.86 | - |
purified subunit B2 | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ribonucleoside diphosphate + reduced thioredoxin | - |
Escherichia coli | 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O | - |
ir | |
ribonucleoside diphosphate + reduced thioredoxin | - |
Escherichia coli overproducing | 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O | - |
ir |
Subunits | Comment | Organism |
---|---|---|
tetramer | alpha2,beta2, 160000 Da subunit B1 and 78000 Da subunit B2, each consisting of 2 identical or similar proteins | Escherichia coli |