Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.17.4.1 extracted from

  • Berglund, O.
    Ribonucleoside diphosphate reductase induced by bacteriophage Tr. I. Purification and characterization (1972), J. Biol. Chem., 247, 7270-7275.
    View publication on PubMed
Search for more literature for 1.17.4.1

Activating Compound

Activating Compound Comment Organism Structure
E. coli thioredoxin reductase absolute requirement Tequatrovirus T4
E. coli thioredoxin reductase enzyme induced in Escherichia coli by infection with bacteriophage T4 Tequatrovirus T4
thioredoxin reductase Escherichia coli enzyme Tequatrovirus T4

General Stability

General Stability Organism
dithiothreitol, 1 mM, stabilizes Tequatrovirus T4
protein concentrations above 5 mg/ml stabilizes Tequatrovirus T4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information effect of different nucleoside triphosphates on Km Tequatrovirus T4

Metals/Ions

Metals/Ions Comment Organism Structure
Iron 2.3 atoms of nonheme iron per molecule Tequatrovirus T4
Mg2+ 5-10 mM, 2-3fold stimulation, not required for enzyme activity Tequatrovirus T4

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 alpha2beta2, 2 * 85000 + 2 * 35000, enzyme induced in Escherichia coli after infection with bacteriophage T4, SDS-PAGE Tequatrovirus T4
85000
-
 alpha2beta2, 2 * 85000 + 2 * 35000, enzyme induced in Escherichia coli after infection with bacteriophage T4, SDS-PAGE Tequatrovirus T4

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
 enzyme induced in Escherichia coli by infection with bacteriophage T4
-

Purification (Commentary)

Purification (Comment) Organism
enzyme induced in Escherichia coli after infection with bacteriophage T4, streptomycin, ammonium sulfate, dATP-Sepharose Tequatrovirus T4

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.09
-
-
 Tequatrovirus T4

Storage Stability

Storage Stability Organism
-70°C, 100 mM potassium phosphate, pH 7.0, 1 mM dithiothreitol, enzyme concentration 4.8 mg/ml, 6 months, no loss of activity Tequatrovirus T4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ribonucleoside diphosphate + reduced thioredoxin dithiothreitol at higher concentrations i.e. 100 mM can partially substitute for reduced T4 thioredoxin, the rate of CDP reduction is 10% of that obtained with the complete system Tequatrovirus T4 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
 ir

Subunits

Subunits Comment Organism
tetramer alpha2beta2, 2 * 85000 + 2 * 35000, enzyme induced in Escherichia coli after infection with bacteriophage T4, SDS-PAGE Tequatrovirus T4

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Tequatrovirus T4

Cofactor

Cofactor Comment Organism Structure
NADPH absolute requirement Tequatrovirus T4
T4 thioredoxin absolute requirement Tequatrovirus T4
T4 thioredoxin enzyme induced in E. coli by infection with bacteriophage T4 Tequatrovirus T4